RNA recognition by the MS2 phage coat protein
RNA recognition by the MS2 phage coat protein
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DOI：
10.1006/smvy.1997.0120
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发表时间：
1997-01-01
期刊：
影响因子：
--
通讯作者：
Uhlenbeck, OC
中科院分区：
文献类型：
--
作者：
Johansson, HE;Liljas, L;Uhlenbeck, OC
MS2 外壳蛋白及其翻译操纵子发夹的相互作用是一种非常明确的 RNA-蛋白质复合物。最近的高分辨率共晶结构成功地解释了许多测量蛋白质-RNA 相互作用对突变蛋白和化学修饰 RNA 的亲和力的生化实验。然而，对 RNA 紧密结合变体的分析表明，游离 RNA 的构象也是亲和力的重要决定因素。 (C) 1997 年学术出版社。
The interaction of MS2 coat protein and its translational operator hairpin is a very well-characterized RNA-protein complex. The recent high-resolution cocrystal structure successfully explains many biochemical experiments measuring the affinity of the protein-RNA interaction for mutant proteins and chemically modified RNAs. However, an analysis of a tight binding variant of the RNA suggests that the conformation of the free RNA is also an important determinant of the affinity. (C) 1997 Academic Press.