Microwave ablation of ex vivo human liver and colorectal liver metastases with a novel 14.5 GHz generator
Microwave ablation of ex vivo human liver and colorectal liver metastases with a novel 14.5 GHz generator
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DOI：
10.3109/02656736.2011.610428
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发表时间：
2012-01-01
影响因子：
3.1
通讯作者：
Malik, Hassan Z.
中科院分区：
文献类型：
--
作者：
Jones, Robert P.;Kitteringham, Neil R.;Malik, Hassan Z.
目的：本研究评估了在离体人肝实质和结直肠肝转移瘤中使用新型高频14.5GHz微波发生器的时间、功率和消融尺寸之间的关系。先前的检查已证明消融区内存在结构正常但无活力的细胞。本研究旨在进一步探讨消融如何影响这些cells，并确认non-viability.Materials and Methods：在离体人肝实质和肿瘤中进行各种时间（10-180 s）和功率（10- 50 W）设置的消融。进行组织学检查以评估细胞解剖结构，同时使用酶组织化学来确认细胞非活力。透射电子显微镜被用来研究这些固定的细胞内的消融的亚细胞结构的影响。初步的蛋白质组学分析也进行了探索微波细胞death.Results的机制：增加时间和功率设置导致了可预测的和可重复的增加消融的大小。在50 W和180 s的应用下，产生的最大消融直径为38.8 mm（+/- 1.3）。消融迅速产生，并且在所有时间和功率设置下，消融保持球形（最长：最短直径< 1.2）。使用苏木精-伊红（H& E）进行的常规组织学分析证实，尽管进行了消融，但细胞解剖结构保存良好。透射电子显微镜显示明显的亚细胞损伤。酶组织化学显示完全没有活力在消融tissue.Conclusions：大的球形消融区可以快速和可重复地实现在离体人肝实质和结直肠肝转移瘤使用14.5GHz的微波发生器。尽管细胞外观保存良好，但消融组织无活力。
Purpose: This study assessed the relationship between time, power and ablation size using a novel high-frequency 14.5GHz microwave applicator in ex vivo human hepatic parenchyma and colorectal liver metastases. Previous examination has demonstrated structurally normal but non-viable cells within the ablation zone. This study aimed to further investigate how ablation affects these cells, and to confirm non-viability.Materials and methods: Ablations were performed in ex vivo human hepatic parenchyma and tumour for a variety of time (10-180 s) and power (10-50W) settings. Histological examination was performed to assess cellular anatomy, whilst enzyme histochemistry was used to confirm cellular non-viability. Transmission electron microscopy was used to investigate the subcellular structural effects of ablation within these fixed cells. Preliminary proteomic analysis was also performed to explore the mechanism of microwave cell death.Results: Increasing time and power settings led to a predictable and reproducible increase in size of ablation. At 50W and 180s application, a maximum ablation diameter of 38.8mm (+/- 1.3) was produced. Ablations were produced rapidly, and at all time and power settings ablations remained spherical (longest: shortest diameter < 1.2). Routine histological analysis using haematoxylin-eosin (H& E) confirmed well preserved cellular anatomy despite ablation. Transmission electron microscopy demonstrated marked subcellular damage. Enzyme histochemistry showed complete absence of viability in ablated tissue.Conclusions: Large spherical ablation zones can be rapidly and reproducibly achieved in ex vivo human hepatic parenchyma and colorectal liver metastases using a 14.5GHz microwave generator. Despite well preserved cellular appearance, ablated tissue is non-viable.