Resveratrol induces apoptosis in human esophageal carcinoma cells.
Resveratrol induces apoptosis in human esophageal carcinoma cells.
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DOI：
10.3748/wjg.v9.i3.408
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发表时间：
2003-03
影响因子：
4.3
通讯作者：
Hai-bo Zhou;Yun Yan;Yani Sun;Ju-ren Zhu
中科院分区：
文献类型：
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作者：
Hai-bo Zhou;Yun Yan;Yani Sun;Ju-ren Zhu
目的探讨白藜芦醇诱导的食道癌细胞凋亡及其与Bcl2、Bax蛋白表达的关系。方法在体外实验中，采用四甲基偶氮唑盐比色法测定细胞生长抑制率。采用透射电子显微镜和TUNEL染色方法，定量、定性地检测白藜芦醇作用前后食管癌细胞系EC-9706的细胞凋亡状态。免疫组织化学染色检测细胞凋亡调控基因Bcl2、Bax的表达。结果白藜芦醇对EC-9706细胞生长有抑制作用，且呈剂量和时间依赖性。白藜芦醇诱导EC-9706细胞发生典型的凋亡特征，包括染色质凝集、染色质新月形成、细胞核碎裂和凋亡小体形成等形态变化。TUNEL法显示，白藜芦醇(10 mmol/L)作用EC-9706细胞24~96h后，AIs随处理时间延长而明显增加(P<；0.05)。免疫组织化学染色显示，白藜芦醇(10 mmol/L)作用EC-9706细胞24~96h后，Bcl2蛋白表达强度随作用时间延长而明显减少(P<0.05)，Bax蛋白表达强度随作用时间延长而明显增加(P<0.05)。结论白藜芦醇具有诱导食管癌细胞凋亡的作用。这种细胞凋亡可能通过下调细胞凋亡调控基因Bcl2和上调细胞凋亡调控基因Bax的表达来实现。
AIM To investigate the apoptosis in esophageal cancer cells induced by resveratrol, and the relation between this apoptosis and expression of Bcl-2 and Bax. METHODS In in vitro experiments, MTT assay was used to determine the cell growth inhibitory rate. Transmission electron microscope and TUNEL staining method were used to quantitatively and qualitively detect the apoptosis status of esophageal cancer cell line EC-9706 before and after the resveratrol treatment. Immunohistochemical staining was used to detect the expression of apoptosis-regulated gene Bcl-2 and Bax. RESULTS Resveratrol inhibited the growth of esophageal cancer cell line EC-9706 in a dose-and time-dependent manner. Resveratrol induced EC-9706 cells to undergo apoptosis with typically apoptotic characteristics, including morphological changes of chromatin condensation, chromatin crescent formation, nucleus fragmentation and apoptotic body formation. TUNEL assay showed that after the treatment of EC-9706 cells with resveratrol (10 mmol/L) for 24 to 96 hours, the AIs were apparently increased with treated time (P<0.05). Immunohistochemical staining showed that after the treatment of EC-9706 cells with resveratrol (10 mmol/L) for 24 to 96 hours, the PRs of Bcl-2 proteins were apparently reduced with treated time (P<0.05) and the PRs of Bax proteins were apparently increased with treated time (P<0.05). CONCLUSION Resveratrol is able to induce the apoptosis in esophageal cancer. This apoptosis may be mediated by down-regulating the apoptosis-regulated gene Bcl-2 and up-regulating the expression of apoptosis-regulated gene bax.