The potential of 3-nitrooxypropanol to lower enteric methane emissions from beef cattle
The potential of 3-nitrooxypropanol to lower enteric methane emissions from beef cattle
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DOI：
10.2527/jas.2014-7573
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发表时间：
2014-10-01
影响因子：
3.3
通讯作者：
Beauchemin, K. A.
中科院分区：
文献类型：
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作者：
Romero-Perez, A.;Okine, E. K.;Beauchemin, K. A.
本研究评估了3-硝基氧丙醇添加到肉牛日粮中是否能减少肠道甲烷（CH 4）排放。研究了3-硝基氧丙醇对日粮消化率、瘤胃发酵和瘤胃微生物的影响。对8头经瘤胃插管的安格斯小母牛（549 +/- 64.3 kg [平均BW +/- SD]）饲喂添加4个水平（0、0.75、2.25和4.50 mg/kg BW）3-硝基氧基丙醇的高饲料饲料（背景饲料）。实验设计为重复4 x 4拉丁方，2组小母牛和4个28天周期。使用代谢室连续3天测量甲烷排放量。当喂食2.5 mg/kg BW的3-硝基氧基丙醇时，观察到自由采食量减少高达5.8%（P = 0.03）。增加3-硝基氧基丙醇的水平线性地（P < 0.001）减少了CH 4，与对照相比，在最高补充水平下CH 4减少了33%（校正为CH 4）。添加3-硝基氧丙醇后，以甲烷形式损失的饲料能量也显著减少（P < 0.001）。随着3-硝基氧丙醇剂量的增加，乙酸的摩尔比例降低（P <0.001），丙酸的摩尔比例增加（P < 0.001），这反过来又导致乙酸与丙酸的比例降低（P < 0.001）。瘤胃内容物中细菌、产甲烷菌的16 S rRNA基因和原虫的18 S rRNA基因的总拷贝数不受3-硝基氧丙醇添加的影响（P >= 0.31）。3-硝基氧丙醇对DM（P = 0.1）的全消化道消化率无影响。使用4.5毫克/公斤体重的3-硝基氧丙醇的肉牛消费的背景饮食是有效的，在减少肠道甲烷排放量，而不会产生负面影响的饮食消化率。
This study evaluated if 3-nitrooxypropanol reduces enteric methane (CH4) emissions when added to the diet of beef cattle. The effects of 3-nitrooxypropanol on related variables including diet digestibility, ruminal fermentation, and ruminal microorganisms were also investigated. Eight ruminally cannulated Angus heifers (549 +/- 64.3 kg [mean BW +/- SD]) were fed a high forage diet (backgrounding diet) supplemented with 4 levels of 3-nitrooxypropanol (0, 0.75, 2.25 and 4.50 mg/kg BW). The experiment was designed as a duplicated 4 x 4 Latin square with 2 groups of heifers and four 28-d periods. Methane emissions were measured during 3 consecutive days using metabolic chambers. Up to a 5.8% reduction in ad libitum DMI was observed when 2.5 mg/kg BW of 3-nitrooxypropanol was fed (P = 0.03). Increasing level of 3-nitrooxypropanol linearly (P < 0.001) reduced CH4, with 33% less CH4 (corrected for DMI) at the highest level of supplementation compared with the control. Feed energy lost as CH4 was also reduced when 3-nitrooxypropanol was supplemented (P < 0.001). Molar proportion of acetate was reduced (P < 0.001) and that for propionate increased (P < 0.001) with increasing dose of 3-nitrooxypropanol, which in turn led to a reduction in the acetate to propionate ratio (P < 0.001). Total copy numbers of 16S ribosomal RNA (rRNA) genes for bacteria, methanogens, and 18S rRNA genes for protozoa in ruminal contents were not affected by 3-nitrooxypropanol supplementation (P >= 0.31). There was no effect of 3-nitrooxypropanol on DM (P = 0.1) digestibility in the total tract. The use of 4.5 mg/kg BW of 3-nitrooxypropanol in beef cattle consuming a backgrounding diet was effective in reducing enteric CH4 emissions without negatively affecting diet digestibility.