Apoptotic and proliferative changes during induced atresia of pre-ovulatory follicles in the rat.
Apoptotic and proliferative changes during induced atresia of pre-ovulatory follicles in the rat.
复制标题
诱导大鼠排卵前卵泡闭锁期间的细胞凋亡和增殖变化。
作者：
A. Durlinger;P. Kramer;B. Karels;J. Grootegoed;J. Uilenbroek;A. P. Themmen
闭锁是一种退行性过程，通过该过程许多卵泡从生长池中移除，涉及卵泡颗粒细胞的凋亡变化。为了鉴定闭锁早期阶段的组织化学标志物，使用体内大鼠模型，其允许以同步和时间顺序研究排卵前卵泡的闭锁。通过用促性腺激素释放激素（GnRH）拮抗剂阻断排卵前促黄体生成激素激增，阻止排卵前卵泡排卵，之后这些卵泡变得闭锁。注射GnRH拮抗剂后27 h发现闭锁的第一个形态学体征（颗粒细胞核固缩）。由于排卵前卵泡以同步的方式逐渐闭锁，该模型提供了一个研究和定义排卵前卵泡未来闭锁标志物的机会。闭锁涉及颗粒细胞的凋亡，因此检查了核小体间DNA片段化。使用末端脱氧核苷酸转移酶介导的dUTP-生物素缺口末端标记法（TUNEL）检测发现，在GnRH拮抗剂治疗后24小时，排卵前卵泡颗粒细胞核小体间DNA片段化的第一个迹象是可检测的。为了找到闭锁的上游标记物，使用5-溴脱氧尿苷（BrdU）标记指数作为增殖的量度。已经在GnRH拮抗剂治疗后14小时，当闭锁的形态学体征尚未出现时，在颗粒细胞中发现BrdU标记指数明显降低。
Atresia, a degenerative process through which many follicles are removed from the growing pool, involves apoptotic changes in the follicular granulosa cells. To identify histochemical markers of early stages of atresia, an in-vivo rat model was used which allowed the study of atresia of pre-ovulatory follicles in a synchronized and chronological order. By blocking the pre-ovulatory luteinizing hormone surge with a gonadotrophin-releasing hormone (GnRH) antagonist, ovulation of the pre-ovulatory follicles is prevented, after which these follicles became atretic. The first morphological sign of atresia (pyknotic granulosa cell nuclei) was found 27 h after injection of GnRH antagonist. Since the pre-ovulatory follicles gradually become atretic in a synchronous fashion, this model provided an opportunity to study and define markers of future atresia in pre-ovulatory follicles. Atresia involves apoptosis of granulosa cells, and therefore internucleosomal DNA fragmentation was examined. Using the terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labelling (TUNEL) assay it was found that the first sign of internucleosomal DNA fragmentation in granulosa cells of pre-ovulatory follicles was detectable 24 h after GnRH antagonist treatment. In order to find an upstream marker of atresia, the 5-bromo-deoxyuridine (BrdU) labelling index was used as a measure of proliferation. Already at 14 h after GnRH antagonist treatment, when morphological signs of atresia were not yet present, a clear decrease in BrdU labelling index was found in the granulosa cells.
登录
查看更多内容
影响因子：
4.8
作者：
S. Chun;H. Billig;J. Tilly;I. Furuta;A. Tsafriri;A. Hsueh
通讯作者：
S. Chun;H. Billig;J. Tilly;I. Furuta;A. Tsafriri;A. Hsueh
大鼠排卵前卵泡闭锁期间脱氧核糖核酸断裂的早期发生。
影响因子：
3.6
作者：
Nahum,R;Beyth,Y;Chun,SY;Hsueh,AJ;Tsafriri,A
通讯作者：
Tsafriri,A
影响因子：
3.6
作者：
S. Quirk;R. Cowan;S. Joshi;K. Henrikson;K. Henrikson
通讯作者：
S. Quirk;R. Cowan;S. Joshi;K. Henrikson;K. Henrikson
影响因子：
4.8
作者：
HUGHES, FM;GOROSPE, WC
通讯作者：
GOROSPE, WC
影响因子：
4.8
作者：
Antti Kaipia;S. Chun;K. Eisenhauer;Aaron J. W. Hsueh
通讯作者：
Antti Kaipia;S. Chun;K. Eisenhauer;Aaron J. W. Hsueh