Deciphering the Molecular Variations of Pine Wood Nematode Bursaphelenchus xylophilus with Different Virulence.
Deciphering the Molecular Variations of Pine Wood Nematode Bursaphelenchus xylophilus with Different Virulence.
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DOI：
10.1371/journal.pone.0156040
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Nian B
中科院分区：
文献类型：
--
作者：
Ding X;Ye J;Lin S;Wu X;Li D;Nian B
松材线虫病是引起松树枯萎病的主要病原，在许多国家造成了巨大的经济损失。据报道，在其原产地存在两种形式的松树木材线虫，即，具有强毒力和弱毒力。然而，人们对这两种形式之间的分子差异知之甚少。为了更好地了解它们的分子变异，分析了3个强毒株和1个弱毒株的转录组和基因组序列。我们发现了238个转录本和84个外显子，显示出显着的变化之间的两个毒力形式。差异表达的转录本和外显子的功能分析表明，不同的毒力菌株表现出不同的线虫生长，繁殖和氧化还原酶活性。此外，我们还在B中检测到少量外显子跳跃事件。木生植物同时，117个SNPs被确定为区分这两种形式的潜在遗传标记。其中4个基因进一步证实发生了等位基因特异性表达，并可能中断了进化保守的B的靶位点。木霉菌miR-47。这些特定的SNP通过包括八个额外的菌株进行实验验证，以确保我们测序结果的有效性。这些结果有助于科学家更好地诊断不同毒力的线虫种类，为松材线虫病的防治提供参考。
Bursaphelenchus xylophilus is the causative agent of pine wilt disease which has caused huge economic losses in many countries. It has been reported that two forms of pine wood nematodes existed in its native region, i.e., with strong virulence and weak virulence. However, little is known about the molecular differences between the two forms. To better understand their molecular variations, transcriptome and genome sequences of three strongly virulent and one weakly virulent strains were analyzed. We found 238 transcripts and 84 exons which showed notable changes between the two virulent forms. Functional analyses of both differentially expressed transcripts and exons indicated that different virulence strains showed dissimilar nematode growth, reproduction, and oxidoreductase activities. In addition, we also detected a small number of exon-skipping events in B. xylophilus. Meanwhile, 117 SNPs were identified as potential genetic markers in distinguishing the two forms. Four of them were further proved to have undergone allele specific expressions and possibly interrupted the target site of evolutionary conserved B. xylophilus miR-47. These particular SNPs were experimentally verified by including eight additional strains to ensure the validity of our sequencing results. These results could help researchers to better diagnose nematode species with different virulence and facilitate the control of pine wilt disease.