Sexual dimorphism and growth hormone induction of murine pheromone-binding proteins.
Sexual dimorphism and growth hormone induction of murine pheromone-binding proteins.
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鼠信息素结合蛋白的性别二态性和生长激素诱导。
DOI：
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发表时间：
1995
影响因子：
3.5
通讯作者：
J. Bishop
中科院分区：
文献类型：
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作者：
D. Johnson;Raya Al;J. Bishop
许多结构非常相似的信息素结合蛋白（主要尿蛋白；MUPs）在小鼠肝脏中合成并迅速随尿液排出。雄性和雌性近交系小鼠表现出不同的MUP表达特征模式。在这里，我们提出了一个详细的研究RNA和蛋白质产物对应的特定MUP基因先前从Balb/c菌株的基因组DNA分离。通过对等效cDNA克隆的体外转录、在网织细胞裂解系统中翻译生成的RNA和等电聚焦，结果表明基因BL1、BS1和BS6的蛋白产物分别为MUP 2a、MUP 2b和MUP 4。MUPs 2a和2b在Balb/c男性尿液和Balb/c男性肝脏总mRNA的翻译产物中均显示丰富。用化学方法合成了分别对BL1和BS1 mRNA具有选择性的两个寡脱氧核苷酸探针oBL1A和oBS1。与这些探针杂交的mRNA （oBL1A mRNA和oBS1 mRNA）在Balb/c和C57BL/6自交系中存在不同的特征水平。在这两种菌株中，雄株表达量均明显高于雌株，且oBS1 RNA的雌雄表达比均高于oBL1A RNA的雌雄表达比。将这些mRNA水平与尿中存在的不同MUP蛋白和肝脏mRNA翻译产物的数量进行比较，表明C57BL/6 oBL1A和oBS1 mRNA编码MUP 2a和MUP 2b以外的蛋白。C57BL/6基因突变纯合的小鼠缺乏生长激素，其MUP基因的转录水平远低于正常小鼠，这表明雄性和雌性均受生长激素诱导。两种不同剂量的GH均可部分诱导MUP基因转录，且诱导的oBL1A mRNA水平高于oBS1 mRNA水平。因此，这些mrna的诱导性与其在雌性中相对于雄性的表达水平之间存在相关性；与oBS1 mRNA相比，oBL1A mRNA在雌性中表达更高，更容易被GH诱导。这表明雄性和雌性的表达模式是由于不同MUP基因的诱导差异以及雄性更强的诱导刺激所致。连续输注GH抑制MUP基因表达。（摘要删节为400字）
A number of structurally very similar pheromone-binding proteins (major urinary proteins; MUPs) are synthesized in mouse liver and rapidly excreted in the urine. Male and female inbred mice display different characteristic patterns of MUP expression. Here we present a detailed study of the RNA and protein products corresponding to specific MUP genes previously isolated from genomic DNA of the Balb/c strain. By in vitro transcription of equivalent cDNA clones, translation of the resulting RNA in the reticulocyte lysate system and isoelectric focusing, the protein products of genes BL1, BS1 and BS6 were shown to be MUP 2a, MUP 2b and MUP 4 respectively. MUPs 2a and 2b were shown to be abundant both in Balb/c male urine and among the translation products of total Balb/c male liver mRNA. Two oligodeoxynucleotide probes, oBL1A and oBS1, selective for BL1 and BS1 mRNA respectively, were chemically synthesized. mRNA that hybridized with these probes (oBL1A mRNA and oBS1 mRNA) was present at different characteristic levels in the Balb/c and C57BL/6 inbred strains. In both strains the level of expression was much higher in males than females and the male/female expression ratio of oBS1 RNA was higher than that of oBL1A RNA. Comparison of these mRNA levels with the amounts of different MUP proteins present in urine and the translation products of liver mRNA indicated that proteins other than MUP 2a and MUP 2b are coded for by the C57BL/6 oBL1A and oBS1 mRNAs. C57BL/6 mice homozygous for the lit mutation are GH deficient and transcribe MUP genes at a level much lower than that obtaining in normal mice of either sex, indicating that transcription is induced by GH in both males and females. When lit/lit mice were treated with GH under two different regimes, MUP gene transcription was partially induced to different degrees and the level of oBL1A mRNA was induced more highly than that of oBS1 mRNA. Thus there exists a correlation between the inducibility of these mRNAs and their level of expression in females relative to males; oBL1A mRNA is both more highly expressed in females and more readily induced by GH than oBS1 mRNA. This suggests that the male and female expression patterns are due to differential inducibility of different MUP genes together with a stronger inducing stimulus in males. GH administered continuously by infusion repressed MUP gene expression.(ABSTRACT TRUNCATED AT 400 WORDS)