Effects of short-term ethanol and nutrition on the hepatic microsomal monooxygenase system in a model utilizing total enteral nutrition in the rat.
Effects of short-term ethanol and nutrition on the hepatic microsomal monooxygenase system in a model utilizing total enteral nutrition in the rat.
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DOI：
10.1111/j.1530-0277.1991.tb00581.x
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发表时间：
1991-08
期刊：
影响因子：
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通讯作者：
M. Ronis;C. Lumpkin;Magnus Ingelman-Sundberg;T. M. Badger
中科院分区：
文献类型：
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作者：
M. Ronis;C. Lumpkin;Magnus Ingelman-Sundberg;T. M. Badger
大多数检查乙醇对肝微粒体单加氧酶系统（HMO）影响的研究都使用了Lieber和DeCarli的流质饮食方案。虽然已经从这个有用的模型中学到了很多东西，但也有一些与营养摄入减少有关的问题。在存在高水平乙醇的情况下食物摄入量减少可能引起至少三种效应：主要乙醇效应、主要营养效应和/或营养缺乏与乙醇之间相互作用引起的效应（即，协同效应）。在本研究中使用了与Tsukomoto和French开发的模型相似的模型，其中将乙醇直接注入胃中作为全肠内营养系统（TEN）的一部分。这保证了正常生长所需的足够营养被消耗掉。选择两种临床相关饮食进行研究。一种饮食非常类似于用于人类患者静脉内喂养的饮食（饮食A），另一种饮食类似于用于患者胃内喂养的饮食（饮食B）。本研究的目的是确定不同饮食对HMO的影响，并确定乙醇是否在促进正常生长率的饮食来源存在下具有明显的影响。检查了单独使用两种流质饮食或将饮食中35%的总热量用乙醇替代8天的十种流质饮食对成年雄性Sprague-Dawley大鼠HMO的影响。HMO底物检查包括睾酮，烷氧基试卤灵，四氯化碳和对硝基苯酚。细胞色素P-450脱辅基蛋白的水平通过Western印迹分析进行了研究。（250字处删节）
The majority of studies examining the effects of ethanol on the hepatic microsomal monooxygenase system, (HMO), have utilized the liquid diet regimen of Lieber and DeCarli. While much has been learned with this useful model, there are some concerns associated with diminished nutrient intake. Decreased food intake in the presence of high levels of ethanol could give rise to at least three effects; primary ethanol effects, primary nutritional effects and/or effects resulting from interactions between nutritional deficiencies and ethanol (i.e., synergistic effects). A model similar to that developed by Tsukomoto and French is used in the current study, in which ethanol is infused directly into the stomach as part of a total enteral nutrition system (TEN). This assured that nutrients sufficient for normal growth were consumed. Two clinically relevant diets were selected for study. One diet is very similar to that used for intravenous feeding of human patients (diet A) and the other similar to that used for intragastric feeding of patients (diet B). The present study was conducted to determine the effects of different diets on HMO and to determine whether ethanol has demonstrable effects in the presence of dietary sources that promote normal growth rates. The effects of the two liquid diets alone or of TEN where 35% of the total calories in the diets were replaced by ethanol for 8 days were examined on HMO of adult male Sprague-Dawley rats. HMO substrates examined included testosterone, the alkoxyresorufins, carbon tetrachloride, and p-nitrophenol. Levels of cytochrome P-450 apoproteins were studied by Western blot analysis.(ABSTRACT TRUNCATED AT 250 WORDS)