Pinhole single-photon emission computed tomography for myocardial perfusion imaging of mice.
Pinhole single-photon emission computed tomography for myocardial perfusion imaging of mice.
复制标题
DOI：
10.1016/s0735-1097(03)00716-2
复制
发表时间：
2003-08
影响因子：
24
通讯作者：
Max C. Wu;D. Gao;R. Sievers;R. Lee;B. Hasegawa;M. Dae
中科院分区：
文献类型：
--
作者：
Max C. Wu;D. Gao;R. Sievers;R. Lee;B. Hasegawa;M. Dae
虽然转基因小鼠已经成为心血管疾病的强大实验模型，但体内表型评估和表征的方法仍然有限，这推动了新的生物测量工具的发展。背景我们开发了一种带有针孔准直器的单光子发射计算机断层扫描系统(Pinhole SPECT)，用于高分辨率的小鼠心血管成像。在这项研究中，我们描述了一种用~(99m)Tc-Sestamibi进行小鼠心肌灌注成像的方法，并论证了从针孔SPECT图像测量心肌灌注缺损区大小的可行性。层析投影图像是通过在配备针孔准直器的固定临床闪烁相机前面沿垂直轴旋转每个鼠标来获得的。BALB/c小鼠(n=15)在冠状动脉左前降支永久结扎后行冠状动脉成像。由此产生的缺陷大小是由短轴图像的周向轮廓测量的。成像完成后，切取心脏切片，获得99mTc-Sestamibi超高分辨率数字放射自显影图像，确定实际梗塞面积。经线性回归分析，缺损区大小的实测值与实际值之间的相关系数为0.001.83。结论针孔断层心肌断层扫描可以定性和定量地评价小鼠的心肌灌注。
ObjectivesAlthough transgenic mice have emerged as powerful experimental models of cardiovascular disease, methods for in vivo phenotypic assessment and characterization remain limited, motivating the development of new instruments for biologic measurement.BackgroundWe have developed a single-photon emission computed tomography system with a pinhole collimator (pinhole SPECT) for high-resolution cardiovascular imaging of mice. In this study, we describe a protocol for myocardial perfusion imaging of mice using technetium-99m (99mTc)-sestamibi and demonstrate the feasibility for measurement of perfusion defect size from pinhole SPECT images.MethodsMice were anesthetized and injected with 370 MBq (10 mCi) of99mTc-sestamibi. Tomographic projection images were acquired by rotating each mouse in a vertical axis in front of a stationary clinical scintillation camera equipped with a pinhole collimator. BALB/c mice (n = 15) were imaged after the permanent ligation of the left anterior descending coronary artery. The resulting defect size was measured from circumferential profiles of short-axis images. After imaging, the hearts were excised and sectioned to obtain ultra-high resolution digital autoradiographs of99mTc-sestamibi, from which the actual infarct size was determined.ResultsReconstructed image quality was equivalent to that obtained for clinical myocardial perfusion imaging. Linear regression analysis produced a correlation coefficient of 0.83 (p < 0.001) between the measured and actual values of the defect size.ConclusionsThese results demonstrate that myocardial perfusion can be characterized qualitatively and quantitatively in mice using pinhole SPECT.