Bioassay of seawater. IV. The determination of dissolved biotin in seawater using 14C uptake by cells of Amphidinium carteri.
Bioassay of seawater. IV. The determination of dissolved biotin in seawater using 14C uptake by cells of Amphidinium carteri.
复制标题
海水生物测定。
DOI：
10.1139/m67-131
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发表时间：
1967
影响因子：
2.8
通讯作者：
S. Silbernagel
中科院分区：
文献类型：
--
作者：
A. Carlucci;S. Silbernagel
本文介绍了一种快速、灵敏的海水生物素测定方法。在仔细控制的条件下，94小时的预孵育后2小时的前沟藻carteri细胞的14 CO2的吸收是成比例的海水中的生物素浓度。细胞数也可用于测量对维生素浓度的反应，但孵育时间延长至144或168 h。 能够测定的生物素浓度范围为0.2-6.0 ng/l。 这些数额大大低于上述数额。
A relatively rapid and sensitive method for the bioassay of seawater for dissolved biotin is described. The uptake of 14CO2 by cells of Amphidinium carteri in 2 h following a 94-h preincubation under carefully controlled conditions is proportional to biotin concentrations in seawater. Cell numbers can also be used to measure response to vitamin concentrations but the incubation time is extended to 144 or 168 h. The range of biotin concentrations capable of being assayed is 0.2–6.0 ng/l. These amounts are considerably less than those previously described.