Modulation of gut mucosal biofilms
Modulation of gut mucosal biofilms
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DOI：
10.1079/bjn20041346
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发表时间：
2005-04-01
影响因子：
3.6
通讯作者：
Blaut, M
中科院分区：
文献类型：
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作者：
Kleessen, B;Blaut, M
不可消化的菊糖型果聚糖，如低聚果糖和高分子量菊糖，已被证明具有改变肠道微生物群组成的能力，其方式是刺激通常被认为是促进健康的微生物群落的成员。双歧杆菌和乳酸杆菌是最常见的目标生物体。关于菊粉型果聚糖对粘膜表面或附近或粘液层中形成粘膜相关生物膜的细菌的组成、代谢和健康相关意义的影响的信息较少。使用接种人类粪便植物群的大鼠作为实验模型，我们发现饮食中的菊糖型果聚糖通过刺激粘膜相关的双歧杆菌以及通过部分减少致病性沙门氏菌肠道亚种来调节肠道微生物群。有益于人体健康。除了粘膜生物膜的变化，菊糖型果聚糖还诱导结肠粘膜的变化，刺激隐窝中的增殖，增加粘蛋白的释放，并改变杯状细胞和上皮粘液层中粘蛋白组分的分布。这些结果表明，菊糖型果聚糖可以稳定肠粘膜屏障。膳食补充这些益生元可以提供一种新的方法来支持粘膜的屏障功能。
Non-digestible inulin-type fructans, such as oligofructose and high-molecular-weight inulin, have been shown to have the ability to alter the intestinal microbiota composition in such a way that members of the microbial community, generally considered as health-promoting, are stimulated. Bifidobacteria and lactobacilli are the most frequently targeted organisms. Less information exists on effects of inulin-type fructans on the composition, metabolism and health-related significance of bacteria at or near the mucosa surface or in the mucus layer forming mucosa-associated biofilms. Using rats inoculated with a human faecal flora as an experimental model we have found that inulin-type fructans in the diet modulated the gut microbiota by stimulation of mucosa-associated bifidobacteria as well as by partial reduction of pathogenic Salmonella enterica subsp. enterica serovar Typhimurium and thereby benefit health. In addition to changes in mucosal biofilms, inulin-type fructans also induced changes in the colonic mucosa stimulating proliferation in the crypts, increasing the release of mucins, and altering the profile of mucin components in the goblet cells and epithelial mucus layer. These results indicate that inulin-type fructans may stabilise the gut mucosal barrier. Dietary supplementation with these prebiotics could offer a new approach to supporting the barrier function of the mucosa.