Novel sodium channel gene mutations in Blattella germanica reduce the sensitivity of expressed channels to deltamethrin
Novel sodium channel gene mutations in Blattella germanica reduce the sensitivity of expressed channels to deltamethrin
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DOI：
10.1016/s0965-1748(01)00122-9
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发表时间：
2002-04-01
影响因子：
3.8
通讯作者：
Dong, K
中科院分区：
文献类型：
--
作者：
Tan, J;Liu, Z;Dong, K
拟除虫菊酯类杀虫剂改变神经系统中电压门控钠通道的正常门控。最近在拟除虫菊酯抗性德国小蠊群体中发现了三种钠通道突变（E434K、C764R、L993F）。在这份报告中，我们表明，L993F突变降低钠通道的敏感性拟除虫菊酯，溴氰菊酯，在爪蟾卵母细胞的5倍。相比之下，单独使用E434 K和C764 R都没有降低通道对溴氰菊酯的敏感性。然而，E434K或C764R与L993F组合使溴氰菊酯敏感性降低100倍。此外，同时存在所有三种突变（KRF）使通道对溴氰菊酯的敏感性降低了500倍。这些突变均未显著影响通道门控。然而，与野生型通道或携带双重或三重突变（KF、RF和KRF）的通道相比，来自单独携带E434 K或C764 R的突变钠通道的钠电流幅度大大降低。这些结果表明，德国小蠊钠通道不敏感性的演变是通过一个初级突变L993F和两个次级突变E434K和C764R的顺序选择实现的，所有三个突变的同时存在显著降低了钠通道对溴氰菊酯的敏感性。(C)2002爱思唯尔科技有限公司版权所有。
Pyrethroid insecticides alter the normal gating of voltage-gated sodium channels in the nervous system. Three sodium channel mutations (E434K, C764R, L993F) were recently identified in pyrethroid resistant German cockroach populations. In this report, we show that the L993F mutation decreased sodium channel sensitivity to the pyrethroid, deltamethrin, by five-fold in Xenopus oocytes. In contrast, neither E434K nor C764R alone decreased channel sensitivity to deltamethrin. However, E434K or C764R combined with L993F reduced deltamethrin sensitivity by 100-fold. Furthermore, concomitant presence of all three mutations (KRF) reduced channel sensitivity to deltamethrin by 500-fold. None of the mutations significantly affected channel gating. However, sodium current amplitudes from the mutant sodium channel carrying either E434K or C764R alone were much reduced compared to those of the wild-type channel or the channel carrying the double or triple mutations (KF, RF and KRF). These results indicated that evolution of sodium channel insensitivity in the German cockroach is achieved by sequential selection of a primary mutation L993F and two secondary mutations E434K and C764R, and concomitant presence of all three mutations dramatically reduced sodium channel sensitivity to deltamethrin. (C) 2002 Elsevier Science Ltd. All rights reserved.