Self-Assembly of Collagen-Mimetic Peptide Amphiphiles into Biofunctional Nanofiber
Self-Assembly of Collagen-Mimetic Peptide Amphiphiles into Biofunctional Nanofiber
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DOI：
10.1021/nn202822f
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发表时间：
2011-10-01
期刊：
影响因子：
17.1
通讯作者：
Tong, Yen Wah
中科院分区：
文献类型：
--
作者：
Luo, Jingnan;Tong, Yen Wah
蛋白质和肽的分子组装是高度特异性的，并且经常发生在生物系统中。胶原蛋白是细胞外基质中含量最丰富的成分，它可以组装成纤维，在细胞粘附和生长中起着重要作用。由于天然胶原蛋白难以修饰，并可能产生致病性和免疫学副作用，其在组织再生中的应用受到限制。因此，胶原蛋白模拟材料的制备在组织再生领域引起了人们的兴趣。已经合成了胶原肽以模拟胶原的一些性质，例如其三螺旋。然而，目前很少有人研究制备人工胶原纤维来模拟其高级结构和生物功能。本工作通过将单一的疏水性Tall与胶原蛋白模拟肽偶联，并添加生物活性的甘氨酸-苯丙氨酸-羟脯氨酸-甘氨酸-谷氨酸-精氨酸（GFOGER），制备了一种新型的胶原蛋白模拟肽两亲物（CPA）。物理研究表明，CPA具有类似胶原的三螺旋构象，并能自组装成单螺旋结构。此外，与整合素特异性GFOGER序列缀合的CPA显示出促进胶原模拟细胞粘附和发育。自组装的肽段具有结构和生物学上模拟天然胶原纤维的能力。我们预计，这种人工胶原纤维具有巨大的潜力，作为胶原模拟材料的组织再生应用。
Molecular assembly of protein and peptide is highly specific and frequently occurs in biological systems. Collagen, which is the most abundant component in extracellular matrix, can assemble into fiber and play an essential role in cell adhesion and growth. Since native collagen Is difficult to modify and can engender pathogenic and Immunological side effects, its application on tissue regeneration is limited. The preparation of collagen-mimetic materials, hence, is gaining interest in the field of tissue regeneration. Collagen peptides have been synthesized to mimic some properties of collagen, such as its triple helix. However, few studies have been done to prepare artificial collagen fiber to mimic its high-level structure and biofunctions. In this work, a novel collagen-mimetic peptide amphiphile (CPA) was prepared by conjugating a single hydrophobic tall with a collagen-mimetic peptide, supplemented with bioactive glycine-phenylalanine-hydroxyproline-glycine-glutamate-arginine (GFOGER). The physical studies indicated that the CPA had a collagen-mimetic triple-helical conformation and was able to self-assemble Into nanofiber. In addition, the CPA conjugated with the Integrin-specific GFOGER sequence was shown to promote collagen-mimetic cell adhesion and development. The self-assembled peptide nanofiber was shown to have the ability to structurally and biologically mimic native collagen fiber. We anticipate that this artificial collagen fiber holds great potential as collagen-mimetic materials for tissue regeneration applications.