Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.)
Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.)
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DOI：
10.1007/s00122-003-1398-6
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发表时间：
2003-11-01
影响因子：
5.4
通讯作者：
Tohme, J
中科院分区：
文献类型：
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作者：
Blair, MW;Pedraza, F;Tohme, J
利用开发的150个微卫星标记对菜豆（Phaseolus vulgaris L.）亲本多态性进行了检测，并确定了100个遗传位点在菜豆综合遗传图谱上的位置。这些单拷贝标记的价值是显而易见的，因为它们能够将两个现有的基于rflp的遗传图谱与为中美洲x安第斯人群（DOR364 x G19833）开发的底图联系起来。基于基因编码和匿名基因组序列对两种微卫星进行了定位。在两个基因池杂交亲本之间，基于基因的基因组微卫星的多态性（46.3%）低于匿名基因组微卫星（64.3%）。大多数微卫星产生单带并检测到单个位点，但4个基于基因的微卫星和3个基因组微卫星产生一致的双带或多带模式并检测到多个位点。在普通豆的11条染色体上均发现了微卫星位点，每条染色体的数量在5 ~ 17个之间，平均每条染色体有10个微卫星位点。碱基图总长度为1720 cM，染色体平均长度为156.4 cM，微卫星位点之间的平均距离为19.5 cM。本文介绍了利用Genbank数据库中的序列开发新型微卫星的情况，以及这些结果对蚕豆遗传定位、数量性状位点分析和标记辅助选择的意义。
A total of 150 microsatellite markers developed for common bean (Phaseolus vulgaris L.) were tested for parental polymorphism and used to determine the positions of 100 genetic loci on an integrated genetic map of the species. The value of these single-copy markers was evident in their ability to link two existing RFLP-based genetic maps with a base map developed for the Mesoamerican x Andean population, DOR364 x G19833. Two types of microsatellites were mapped, based respectively on gene-coding and anonymous genomic-sequences. Gene-based microsatellites proved to be less polymorphic (46.3%) than anonymous genomic microsatellites (64.3%) between the parents of two intergenepool crosses. The majority of the microsatellites produced single bands and detected single loci, however four of the gene-based and three of the genomic microsatellites produced consistent double or multiple banding patterns and detected more than one locus. Microsatellite loci were found on each of the 11 chromosomes of common bean, the number per chromosome ranging from 5 to 17 with an average of ten microsatellites each. Total map length for the base map was 1,720 cM and the average chromosome length was 156.4 cM, with an average distance between microsatellite loci of 19.5 cM. The development of new microsatellites from sequences in the Genbank database and the implication of these results for genetic mapping, quantitative trait locus analysis and marker-assisted selection in common bean are described.