Preparation and characterization of composite hydrogels based on crosslinked hyaluronic acid and sodium alginate
Preparation and characterization of composite hydrogels based on crosslinked hyaluronic acid and sodium alginate
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DOI：
10.1002/app.41898
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发表时间：
2015-05-15
影响因子：
3
通讯作者：
Wang, Qiang
中科院分区：
文献类型：
--
作者：
Chen, Yong-Hao;Li, Jun;Wang, Qiang
本研究通过透明质酸（HA）和海藻酸钠（SAL）的交联制备了一种新型复合水凝胶，该复合水凝胶具有更好的泡孔结构和力学性能。通过傅里叶变换红外光谱法证实了我们预期形成的水凝胶中的酰胺键（共价键）。水凝胶具有大于100 μ m的孔径，并通过扫描电子显微镜观察。质地剖面分析表明，水凝胶的硬度增强的聚合物的浓度的增加，但它随着HA/SAL摩尔比的增加而下降。溶胀能力随着聚合物浓度和1-乙基-3-（3-二甲基氨基丙基）-1-碳二亚胺盐酸盐（EDC）/HA摩尔比的增加而降低，并且通过HA/SAL摩尔比的增加而增强。对透明质酸酶的抗性与水凝胶中HA的比例呈负相关，与EDC/HA摩尔比呈正相关。鉴于我们生产的改进的物理化学性质，这些新型水凝胶可能具有应用于组织工程支架的潜力。(c)2015 Wiley Periodicals，Inc. J.应用聚合物Sci. 2015，132，41898。
In this study, a novel composite hydrogel with improved cellular structure and mechanical properties was prepared by the crosslinking of hyaluronic acid (HA) and sodium alginate (SAL). The amide linkages (covalent bonds) in the hydrogel that we expected to form were confirmed by Fourier transform infrared spectroscopy. The hydrogels had a pore size larger than 100 m and were observed by scanning electron microscopy. Texture profile analysis indicated that the hardness of the hydrogels was enhanced by an increase in the polymer's concentration, but it declined with an increase in the HA/SAL molar ratio. The swelling capacity was reduced with increases in the polymer's concentration and the 1-ethyl-3-(3-dimethyl aminopropyl)-1-carbodiimide hydrochloride (EDC)/HA molar ratio, and it was enhanced by an increase in the HA/SAL molar ratio. The resistance against hyaluronidase was negatively correlated with the proportion of HA in the hydrogels and positively correlated with the EDC/HA molar ratio. Given the improved physicochemical properties that we produced, these novel hydrogels may have the potential to be applied in tissue engineering scaffolding. (c) 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 41898.