Understanding Structure: A Computer-Based Macromolecular Biochemistry Lab Activity
Understanding Structure: A Computer-Based Macromolecular Biochemistry Lab Activity
复制标题
理解结构：基于计算机的高分子生物化学实验室活动
DOI：
10.1021/acs.jchemed.6b00464
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发表时间：
2017
影响因子：
3
通讯作者：
K. McLaughlin
中科院分区：
文献类型：
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作者：
K. McLaughlin
生物学和化学专业的本科生会遇到蛋白质结构的图像，这些图像可以通过X射线晶体学来解决，但他们通常没有能力在教育中解释和使用这些图像。一个简单的基于计算机的实验室活动介绍和描述在这里，向学生介绍X射线晶体图像的起源，并允许他们体验蛋白质模型构建的基础知识，通过使用溶菌酶。实验活动还指导学生研究溶菌酶作为G蛋白偶联受体溶液中结晶助剂的作用。学生学习使用模型构建和可视化程序，Coot和PyMOL，沿着与RCSB蛋白质数据库，以获得，使用和解释电子密度图与结构文件，以评估蛋白质模型。
Undergraduates in biology and chemistry encounter images of protein structures, solved by X-ray crystallography, but are often ill equipped to interpret and use these images in their education. A simple computer-based lab activity is presented and described here that introduces students to the origin of X-ray crystallographic images and allows them to experience the basics of protein model building, through the use of lysozyme. The lab activity also directs students to examine lysozyme’s role as a crystallization aid in the solution of a G-protein-coupled receptor. Students learn to use model building and visualization programs, Coot and PyMOL, along with the RCSB Protein Data Bank, to obtain, use and interpret electron density maps with structure files to assess a protein model.