A cotton gene encoding a plasma membrane aquaporin is involved in seedling development and in response to drought stress.
A cotton gene encoding a plasma membrane aquaporin is involved in seedling development and in response to drought stress.
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DOI：
10.1093/abbs/gms096
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发表时间：
2013-02
影响因子：
3.7
通讯作者：
Jie Zhang;Deng-Di Li;Dan Zou;Fang Luo;Xiulan Wang;Yong Zheng;Xue-Bao Li
中科院分区：
文献类型：
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作者：
Jie Zhang;Deng-Di Li;Dan Zou;Fang Luo;Xiulan Wang;Yong Zheng;Xue-Bao Li
棉花是世界上最重要的纺织作物，在其生长季节(夏季)经常遭受干旱、内涝等非生物胁迫，其产量受到不利因素的严重限制。为了研究该植物对非生物胁迫的分子适应机制，在棉花中克隆了一个编码质膜内源蛋白(PIP)的基因，命名为GhPIP2；7.定量逆转录聚合酶链式反应分析表明，GhPIP2；7在子叶和叶片中优先表达，干旱处理后GhPIP2；7在叶片中表达上调。GhPIP2；7启动子驱动的GUS基因在5~10天龄的转基因拟南芥幼苗叶片中有较强的表达，但随着幼苗的进一步发育，GUS活性逐渐减弱。甘露醇对GhPIP2；7启动子活性也有明显的诱导作用。此外，与对照相比，高表达GhPIP2；7的酵母细胞表现出相对更高的抗旱性。GhPIP2；7在拟南芥中的过表达提高了植物对干旱胁迫的耐受性。综上所述，这些数据表明GhPIP2；7基因可能参与了叶片的发育和对干旱胁迫的响应。
Cotton (Gossypium hirsutum), the most important textile crop worldwide, often encounters abiotic stress such as drought and waterlog during its growth season (summer), and its productivity is significantly limited by adverse factors. To investigate the molecular adaptation mechanisms of this plant species to abiotic stress, a gene encoding the plasma membrane intrinsic protein (PIP) was isolated in cotton, and designated as GhPIP2;7. Quantitative reverse transcriptase polymerase chain reaction analysis indicated that GhPIP2;7 was preferentially expressed in cotyledons and leaves, and its expression was up-regulated in leaves after drought treatments. Strong expression of GUS gene driven by GhPIP2;7 promoter was detected in leaves of 5- to 10-day-old transgenic Arabidopsis seedlings, but GUS activity gradually became weak as the seedlings further developed. GhPIP2;7 promoter activity was also remarkably induced by mannitol treatment. Furthermore, yeast cells over-expressing GhPIP2;7 displayed relatively higher drought tolerance, compared with controls. Over-expression of GhPIP2;7 in Arabidopsis enhanced plant tolerance to drought stress. Collectively, these data suggested that GhPIP2;7 gene may be involved in leaf development and in response to drought stress.