Reduced soluble CD14 levels in amniotic fluid and breast milk are associated with the subsequent development of atopy, eczema, or both
Reduced soluble CD14 levels in amniotic fluid and breast milk are associated with the subsequent development of atopy, eczema, or both
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DOI：
10.1067/mai.2002.123535
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发表时间：
2002-05-01
影响因子：
14.2
通讯作者：
Warner, JO
中科院分区：
文献类型：
--
作者：
Jones, CA;Holloway, JA;Warner, JO
背景：在生命早期暴露于各种微生物制品可降低特应性的风险。这样的曝光诱导下调T（H）2过敏偏向的反应，通过模式识别分子，如CD 14，LPS receptor.Objective：我们试图确定是否婴儿和母亲的可溶性CD 14（sCD 14）的水平与atopic outcomes of infants.Methods：血浆中的sCD 14水平，羊水，母乳中测定了一个特定的ELISA在不同的队列。Toll样受体在胎儿肠道中的表达进行了检查，通过使用RT-PCR.Results：可溶性CD 14水平在胎儿发育和出生后增加，达到成人水平的4个月左右的年龄，从6个月的年龄与成人水平的超调。与低风险儿童相比，高风险儿童出生时血浆sCD 14水平无差异。在妊娠中期（16-17周），当孩子随后出现特应性时，羊水sCD 14水平显著降低（P <0.05）。产后3个月收集的母乳中可溶性CD 14水平在6个月大的湿疹儿童中显著较低，无论他们是否为特应性（P = .003）。转录Toll样受体4，这将使跨膜信号的LPS/sCD 14复合物，表达在胎儿肠道和skin.Conclusion：暴露于胎儿和新生儿胃肠道sCD 14水平降低与特应性，湿疹，或两者兼而有之的发展。因此，外源性供应的sCD 14可能会影响免疫反应的局部和全身在生命早期，从而影响疾病的结果。
Background: Exposure to various microbial products in early life reduces the risk of atopy. Such exposure induces down-regulation of T(H)2 allergy-biased responses by means of pattern recognition molecules, such as CD14, an LPS receptor.Objective: We sought to determine whether infant and maternal levels of soluble CD14 (sCD14) are associated with the atopic outcomes of infants.Methods: Levels of sCD14 in plasma, amniotic fluid, and breast milk were measured with a specific ELISA in different cohorts. Expression of toll-like receptors in the fetal gut was examined by using RT-PCR.Results: Soluble CD14 levels increased during fetal development and postnatally, attaining adult levels by around 4 months of age, with an overshoot of adult levels from 6 months of age. There was no difference in plasma sCD14 levels at birth of children with a high compared with those with a low risk of development of atopy. Amniotic fluid sCD14 levels at midgestation (16-17 weeks) were significantly lower when the child was subsequently atopic (P < .05). Soluble CD14 levels in breast milk collected 3 months postpartum were significantly lower in children with eczema at 6 months of age, irrespective of whether they were atopic (P = .003). Transcripts for toll-like receptor 4, which would enable transmembrane signaling for LPS/sCD14 complexes, were expressed within fetal gut and skin.Conclusion: Exposure to reduced levels of sCD14 in the fetal and neonatal gastrointestinal tract is associated with the development of atopy, eczema, or both. Thus the exogenous supply of sCD14 might influence immunologic reactivity both locally and systemically in early life and thereby influence disease outcome.