Capsaicin-sensitive afferent fibers contribute to gastric mucosal blood flow response to electrical vagal stimulation.
Capsaicin-sensitive afferent fibers contribute to gastric mucosal blood flow response to electrical vagal stimulation.
复制标题
辣椒素敏感的传入纤维有助于胃粘膜血流对迷走神经电刺激的反应。
DOI：
10.1152/ajpgi.1990.259.6.g1037
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Guth,PH
中科院分区：
文献类型：
--
作者：
Thiefin,G;Raybould,HE;Leung,FW;Tache,Y;Guth,PH
电刺激外周迷走神经可通过未知机制引起胃粘膜血流量（GMBF）的非胆碱能增加。本研究的目的是：1）研究在长时间迷走神经电刺激期间GMBF的增加是否涉及与酸分泌增加不同的机制; 2）确定传入纤维的逆向激活是否有助于迷走神经电刺激的血管或分泌反应。电迷走神经刺激（40 V，6 Hz，2 ms）施加30分钟的远侧切断端的腹侧迷走神经下显着增加胃酸分泌和GMBF测量氢气清除。阿托品（0.15 mg/kg iv）或奥美拉唑（10 mumol/kg iv）完全消除迷走神经电刺激的分泌反应，而GMBF仍显着增加。预处理与神经周围应用的感觉神经毒素辣椒素的两个颈部迷走神经显着减少了48%的GMBF的增加，但不是胃酸分泌;阿托品完全取消了剩余的辣椒素治疗的大鼠血管反应。这些结果表明，长时间的电迷走神经刺激诱导GMBF的持续增加，部分独立于增强的酸分泌和血管反应的非胆碱能部分介导的辣椒素敏感的迷走神经传入纤维。
Electrical stimulation of the peripheral vagus produces a noncholinergic increase in gastric mucosal blood flow (GMBF) via unknown mechanisms. The purpose of this study was 1) to investigate whether a portion of the increase in GMBF during prolonged electrical vagal stimulation involves a mechanism separate from augmented acid secretion and 2) to determine whether antidromic activation of afferent fibers contributes to the vascular or secretory responses to electrical vagal stimulation. Electrical vagal stimulation (40 V, 6 Hz, 2 ms) applied for 30 min to the distal cut end of the subdiaphragmatic ventral vagus significantly increased gastric acid secretion and GMBF measured by hydrogen gas clearance. Atropine (0.15 mg/kg iv) or omeprazole (10 mumol/kg iv) completely abolished the secretory response to electrical vagal stimulation, while a significant increase in GMBF remained. Pretreatment with perineural application of the sensory neurotoxin capsaicin to both cervical vagi significantly reduced by 48% the increase in GMBF but not gastric acid secretion; atropine completely abolished the remaining vascular response in capsaicin-treated rats. These results suggest that prolonged electrical vagal stimulation induces a sustained increase in GMBF partially independent of augmented acid secretion and that the noncholinergic portion of the vascular response is mediated by capsaicin-sensitive vagal afferent fibers.