Genetics of human cell lines. III. Incorporation of 5-bromo- and 5-iododeoxyuridine into the deoxyribonucleic acid of human cells and its effect on radiation sensitivity.
Genetics of human cell lines. III. Incorporation of 5-bromo- and 5-iododeoxyuridine into the deoxyribonucleic acid of human cells and its effect on radiation sensitivity.
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人类细胞系的遗传学。 iii。将5-溴和5-二羟基尿苷掺入人类细胞的脱氧核糖核酸及其对辐射敏感性的影响。
DOI：
10.1084/jem.112.3.509
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发表时间：
1960-09-01
期刊：
影响因子：
--
通讯作者：
SZYBALSKI W
中科院分区：
文献类型：
--
作者：
DJORDJEVIC B;SZYBALSKI W
人细胞系D98S可在高达3 x 10 - 5 M 5-溴脱氧尿苷（BUDR）存在下无限期培养，而不会丧失细胞活力。在此期间，BUDR被整合到DNA分子的两条链中，取代多达45%的胸苷，从而使细胞对紫外线和X射线高度敏感。在5-碘脱氧尿苷（IUDR）存在下生长有限时间的细胞变得UV敏化，而用IUDR长时间培养导致细胞活力丧失。BUDR标记的性质允许证明：（a）人DNA以"双保守"方式复制;（B）BUDR处理的细胞的放射增敏程度取决于DNA是否仅在一条链（"单线"）或两条链（"双线"）中被取代;（c）在蛋白质合成的部分抑制期间产生功能性人DNA。讨论了这种新的合理的放射增敏原理对肿瘤性疾病放射治疗的潜在适用性。
The human cell line D98S can be cultivated indefinitely in the presence of up to 3 x 10–5 M 5-bromodeoxyuridine (BUDR), without loss of cell viability. During this time, BUDR is incorporated into both strands of the DNA molecules, replacing up to 45 per cent of the thymidine and thereby rendering the cells highly sensitive to UV light and to x-rays. Cells grown for a limited period of time in the presence of 5-iododeoxyuridine (IUDR) become UV-sensitized, while prolonged cultivation with IUDR results in the loss of cell viability. The properties of the BUDR label permitted the demonstration that: (a) human DNA replicates in a "semiconservative" manner; (b) the degree of radiosensitization of BUDR-treated cells depends on whether the DNA has been substituted in one strand only ("unifilarly") or in both strands ("bifilarly"); (c) functional human DNA is produced during partial inhibition of protein synthesis. The potential applicability of this new rational principle of radiosensitization to the radiotherapy of neoplastic diseases is discussed.