Expression of amelin and trauma-induced dentin formation
Expression of amelin and trauma-induced dentin formation
复制标题
amelin 的表达和创伤诱导的牙本质形成
DOI：
10.1007/s00784-001-0139-y
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发表时间：
2002
影响因子：
3.4
通讯作者：
L. Hammarström
中科院分区：
文献类型：
--
作者：
A. Spahr;S. Lyngstadaas;I. Slabý;B. Haller;C. Boeckh;F. Tsoulfidou;L. Hammarström
抽象的。据最近的研究，釉蛋白（成釉蛋白，鞘蛋白）表达在年轻的成牙本质细胞在牙本质形成过程中的启动。本研究的目的是研究amelin是否也表达在创伤诱导的修复性牙本质形成的发病。手术取出5日龄大鼠下颌发育中的第一磨牙，将其牙髓组织与牙本质内表面短暂分离并立即重新定位。然后将牙齿重新植入牙槽。术后0、2、4、6、8、12或14天处死动物，对实验牙进行组织学和釉蛋白免疫组化评价。术后2、4、6和8天，实验牙中分离和创伤的成牙本质细胞表现出越来越多的变性和细胞内结构丢失的迹象。在术后第6天和第8天，免疫组化显示，在创伤成牙本质细胞层的amelin强染色。再植后12天和14天，只有坏死的细胞残留的创伤成牙本质细胞是可辨别的。在这个阶段，没有釉蛋白可以检测到免疫染色。成牙本质细胞残余物周围有一大片无组织的矿化组织。在无组织的组织的牙髓侧，观察到一个新的，高度组织化的管状修复性牙本质层，邻接新形成的前牙本质的柱状成牙本质细胞样细胞。结果表明，创伤诱导的修复性牙本质形成的启动模仿的初级牙本质的形成和釉蛋白似乎参与这两个过程中，可能作为一个信号分子。
Abstract. According to recent studies, amelin (ameloblastin, sheathlin) is expressed in young odontoblasts at the initiation of dentin formation during odontogenesis. The purpose of the present investigation was to study whether amelin is also expressed at the onset of trauma-induced reparative dentin formation. The mandibular developing first molars of 5-day-old rats were surgically taken out, and their pulp tissue briefly separated from the inner dentin surface and immediately repositioned. Then the teeth were re-implanted in their alveoli. At 0, 2, 4, 6, 8, 12 or 14 days after surgery, the animals were sacrificed and the experimental teeth evaluated by histology and immunohistochemistry for amelin. At 2, 4, 6 and 8 days after surgery, the detached and traumatized odontoblasts in the experimental teeth exhibited increasing signs of degeneration and loss of intracellular structures. At days 6 and 8 after surgery, immunohistochemistry revealed a strong staining for amelin in the traumatized odontoblastic layer. Twelve and 14 days after replantation, only necrotic cell remnants of the traumatized odontoblasts were discernible. At this stage, no amelin could be detected by immunostaining. A wide zone of an unorganized mineralized tissue surrounded the odontoblastic cell remnants. On the pulpal side of the unorganized tissue, a new, highly organized tubular reparative dentin layer was observed, bordered by columnar odontoblast-like cells abutting on newly formed predentin. The results indicate that the initiation of trauma-induced reparative dentin formation mimics that of primary dentin formation and that amelin seems to be involved in both processes, possibly as a signaling molecule.
影响因子：
3.2
作者：
Uchida, T;Murakami, C;Takahashi, O
通讯作者：
Takahashi, O