Induction of micronuclei in CHO cells by bleomycin but not by X-irradiation is decreased by treatment with HMG-CoA reductase inhibitors
Induction of micronuclei in CHO cells by bleomycin but not by X-irradiation is decreased by treatment with HMG-CoA reductase inhibitors
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DOI：
10.1269/jrr.46.191
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发表时间：
2005-06-01
影响因子：
2
通讯作者：
Watanabe, M
中科院分区：
文献类型：
--
作者：
Iwashita, J;Kodama, S;Watanabe, M
我们研究了 3-羟基-3-甲基戊二酰辅酶 A (HMG-CoA) 还原酶抑制剂普伐他汀和氟伐他汀对中国仓鼠卵巢细胞中电离辐射或博来霉素诱导微核的影响，以评估这些抑制剂的自由基清除能力。结果表明，普伐他汀和氟伐他汀在治疗前和治疗后均对X射线诱导微核没有影响。相比之下，两种药物在同时治疗或治疗后均能有效降低博莱霉素诱导的微核频率，但在治疗前则无效果。这表明这两种 HMG-CoA 还原酶抑制剂的自由基清除能力根据自由基的来源（例如 X 射线或博来霉素）而有所不同，即使这两种药物在相同的细胞毒性剂量下进行比较也是如此。我们的结果表明，普伐他汀和氟伐他汀都具有清除某些类型自由基并保护细胞免受氧化应激的能力。
We investigated the effect of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors, pravastatin and fluvastatin, on the induction of micronuclei by ionizing radiation or bleomycin in Chinese hamster ovary cells in order to assess the radical-scavenging ability of these inhibitors. The results indicated that both pravastatin and fluvastatin had no effect on the induction of micronuclei by X-irradiation when they were applied for either pre-treatment or post-treatment. In contrast, both drugs effectively reduced the frequency of bleomycin-induced micronuclei when they were applied for simultaneous treatment or post-treatment, but not for pre-treatment. This indicates that the radical-scavenging ability of these two HMG-CoA reductase inhibitors differs according to the origins of the radicals - e.g., X-rays or bleomycin - even when the two drugs are compared at an equivalent cytotoxic dose. Our results suggest that both pravastatin and fluvastatin have the ability to scavenge certain types of radicals and to protect cells against oxidative stress.