Immunologically relevant strain polymorphism in the Amastigote Surface Protein 2 of Trypanosoma cruzi
Immunologically relevant strain polymorphism in the Amastigote Surface Protein 2 of Trypanosoma cruzi
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DOI：
10.1016/j.micinf.2007.04.006
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发表时间：
2007-07-01
影响因子：
5.8
通讯作者：
Rodrigues, Mauricio M.
中科院分区：
文献类型：
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作者：
Claser, Carla;Espindola, Noeli Maria;Rodrigues, Mauricio M.
多项证据表明，克氏锥虫无鞭毛体表面蛋白 2 (ASP-2) 是感染期间免疫的重要目标。基于此，我们认为评价其菌株多态性具有重要意义。最初，我们观察到所有测试的寄生虫菌株的无鞭毛体中都存在保守的交叉反应表位。此外，从 CL-Brener、Tulahuen、Colombia 和 G 品系的无鞭毛体 cDNA 中分离的基因的预测氨基酸序列与先前描述的 ASP-2 的 C 基因显示出高度的同一性 (> 80%)。出乎意料的是，Sylvio X10/4 和 G 菌株表达了一种新的 ASP-2 亚型，与之前描述的基因具有有限的同一性，但相互比较时具有高度的同一性。免疫学研究证实了代表 ASP-2 不同亚型的重组蛋白之间存在交叉反应表位。然而，用G株表达的新亚型asp-2基因对小鼠进行基因疫苗接种，未能提供与Y株或CL-Brener株的asp-2基因相同程度的针对Y株寄生虫攻击的保护性免疫。总之，我们发现很少有菌株可以表达不同的 ASP-2 亚型，而这些亚型可能不共享交叉保护表位。 (c) 2007 年 Elsevier Masson SAS。版权所有。
Several evidences suggest that the Amastigote Surface Protein-2 (ASP-2) of Trypanosonia cruzi is an important target for immunity during infection. Based on this, we considered it important to evaluate its strain polymorphism. Initially, we observed the presence of conserved cross-reactive epitopes in amastigotes of all parasite strains tested. In addition, the predicted amino acid sequences of the genes isolated from the cDNA of amastigotes of CL-Brener, Tulahuen, Colombian and G strains displayed a high degree of identity (> 80%) to the previously described C Genes of ASP-2. Unexpectedly, Sylvio X10/4 and G strains expressed a new isoform of ASP-2 with limited identity to the previously described genes, but with a high degree of identity when compared to each other. Immunological studies confirmed the presence of cross-reactive epitopes between recombinant proteins representing the different isoforms of ASP-2. However, the genetic vaccination of mice with the new isoform of asp-2 gene expressed by the G strain failed to provide the same degree of protective immunity to a challenge by parasites of the Y strain as did asp-2 genes of Y or CL-Brener strains. In summary, we found that few strains can express different isoforms of ASP-2 which may not share cross-protective epitopes. (c) 2007 Elsevier Masson SAS. All rights reserved.