Chicken Y-box proteins chk-YB-1b and chk-YB-2 repress translation by sequence-specific interaction with single-stranded RNA
Chicken Y-box proteins chk-YB-1b and chk-YB-2 repress translation by sequence-specific interaction with single-stranded RNA
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DOI：
10.1042/0264-6021:3480297
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发表时间：
2000-06-01
影响因子：
4.1
通讯作者：
Guntaka, RV
中科院分区：
文献类型：
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作者：
Swamynathan, SK;Nambiar, A;Guntaka, RV
Y-Box蛋白是一个多功能蛋白家族，在基因表达的转录和翻译调控中具有广泛的活性。早些时候，我们已经报道了chk-YB-2参与Rous肉瘤病毒长末端重复序列的转录调控，以及chk-YB-1b参与α 1（I）胶原基因的转录调控。在这里，我们研究了chk-YB-2和chk-YB-1b在RNA代谢中的潜在作用。我们报告说，chk-YB-2和chk-YB-1b主要定位于细胞质中，它们都可以结合单链RNA的序列特异性和可逆的方式。这两个蛋白质的冷休克结构域、N端富含脯氨酸结构域以及C端的酸性和碱性氨基酸交替簇是它们与RNA结合所必需的。此外，我们证明，这两个蛋白质抑制翻译在体外，结合RNA是需要这种抑制，这些结果的意义进行了讨论。
Y-Box proteins comprise a large family of multifunctional proteins with a wide spectrum of activities in both transcription and translational regulation of gene expression. Earlier, we have reported on the involvement of chk-YB-2 in transcriptional regulation of Rous sarcoma virus long terminal repeats and the involvement of chk-YB-1b in transcriptional regulation of alpha 1(I) collagen genes. Here, we have investigated the potential role of chk-YB-2 and chk-YB-1b in RNA metabolism. We report that chk-YB-2 and chk-YB-1b are localized predominantly in the cytoplasm and that they both can bind single-stranded RNA in a sequence-specific and reversible manner. Well-conserved cold-shock domain, N-terminal proline-rich domain and the alternating clusters of acidic and basic amino acids located in the C-terminal ends of these two proteins were all found to be necessary for their RNA-binding ability. Further, we demonstrate that these two proteins inhibit translation in vitro and that binding to RNA is required for this inhibition, The significance of these results is discussed.