Pliable synaptic fidelity and excitatory inter-synaptic crosstalk in the intact brain
Pliable synaptic fidelity and excitatory inter-synaptic crosstalk in the intact brain
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DOI：
10.1101/2022.03.04.483016
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发表时间：
2022-03
期刊：
影响因子：
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通讯作者：
James P. Reynolds;Thomas P. Jensen;Sylvain Rama;Kaiyu Zheng;L. Savtchenko;D. Rusakov
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作者：
James P. Reynolds;Thomas P. Jensen;Sylvain Rama;Kaiyu Zheng;L. Savtchenko;D. Rusakov
神经回路中的记忆形成可能涉及突触效能和细胞内在兴奋性的变化，但这一过程如何在活体大脑中展开仍然是一个谜。在这里，我们采用多路复用成像的遗传编码的指标，谷氨酸和Ca2+在小鼠桶皮质检测增加保真度加上减少兴奋的丘脑皮质连接，经历胡须刺激诱导的LTP。高分辨率成像显示，胡须刺激触发兴奋性突触活动，产生突触外谷氨酸瞬变，到达目标皮层区域的大部分相邻突触。我们的研究结果为理解突触连接体的基本可塑性特征铺平了道路，同时揭示了完整大脑中细胞间体积传递的神经元信号传导的重要组成部分。一句话总结感觉刺激LTP增加保真度，同时减少单个丘脑皮层连接的兴奋，这些连接产生空间交叉的谷氨酸放电
Memory formation in neural circuits may involve changes in synaptic efficacy and in cell intrinsic excitability, yet how this process unfolds in the living brain has remained elusive. Here, we employed multiplexed imaging of genetically encoded indicators of glutamate and Ca2+ in mouse barrel cortex to detect increased fidelity coupled with reduced excitation of thalamocortical connections that undergo whisker-stimulation induced LTP. High-resolution imaging revealed that whisker stimuli trigger excitatory synaptic activity that generates extrasynaptic glutamate transients reaching the bulk of neighbouring synapses in the target cortical area. Our findings pave the way to understanding basic plasticity features of the synaptic connectome while revealing a significant component of volume-transmitted glutamatergic signalling among cells in the intact brain. One Sentence Summary Sensory-stimulation LTP increases fidelity while reducing excitation at individual thalamocortical connections which generate spatially intersecting glutamate discharges