DNA marker analysis of flowering time, semi-dwarf, fertility restorer, and brown midrib genes in sorghum (Sorghum bicolor [L.] Moench)
DNA marker analysis of flowering time, semi-dwarf, fertility restorer, and brown midrib genes in sorghum (Sorghum bicolor [L.] Moench)
复制标题
DOI：
10.1111/grs.12343
复制
发表时间：
2021-07-26
影响因子：
1.3
通讯作者：
Kasuga, Sigemitsu
中科院分区：
文献类型：
--
作者：
Kawahigashi, Hiroyuki;Yonemaru, Jun-ichi;Kasuga, Sigemitsu
本研究开发了开花时间基因（Ma 1/SbPRR 37、Ma 2/SbEHD 1、Ma 3/SbPhyB、Ma 4/SbCO、Ma5/SbPhyC和Ma 6/SbGHD 7）、半矮秆基因（dw 1和dw 2）、育性恢复基因（Rf 1、Rf 2、Rf 5和部分A1线粒体序列）、细胞质-核雄性不育基因（CMS）和棕色中脉基因（bmr-2、bmr-6和bmr-18）的DNA标记。这些标记和以前发表的DNA标记dw 3被用来分析高粱品种在育种计划中使用的日本和传统的地方品种从国家农业科学研究所（NIAS）高粱核心收藏。利用该DNA标记可以有效地检测到供试品种中的农艺学上有用的突变。在高粱品种中检测到大多数开花和半矮秆突变类型，但在NIAS收集中仅检测到SbEHD 1和SbPhyB的显性开花等位基因。在NIAS收集的材料中很少检测到育性恢复系和CMS。农艺学上有用的性状，如早花和半矮化成为结合在现代品种的表型评价收获适应温带。在F-1代杂交种选育中，引入恢复系和不育系。这些表型通常是隐性的，因此有关等位基因的信息将有助于高粱育种。我们希望这些DNA标记能促进和提高F-1高粱育种计划的效率。
DNA markers for the genes for flowering time (Ma1/SbPRR37, Ma2/SbEHD1, Ma3/SbPhyB, Ma4/SbCO, Ma5/SbPhyC, and Ma6/SbGHD7), semi-dwarfism (dw1 and dw2), fertility restorers (Rf1, Rf2, Rf5, and partial A1 mitochondrial sequence) and cytoplasmic-nuclear male sterile (CMS), and brown midrib (bmr-2, bmr-6, and bmr-18) were developed in this study. These markers and previously published DNA markers for dw3 were used to analyze sorghum cultivars used in breeding programs in Japan and traditional landraces from the National Institute of Agricultural Sciences (NIAS) sorghum core collection. Agronomically useful mutations were effectively detected in the cultivars by the DNA markers. Most of the mutation types for flowering and semi-dwarfism were detected in sorghum cultivars, but only the dominant-type allele of SbEHD1 and SbPhyB for flowering was detected in the NIAS collection. Fertility restorers and CMS were rarely detected in NIAS collection. Agronomically useful traits such as early flowering and semi-dwarfism became combined in the modern cultivars by phenotypic evaluation for the adaptation of harvesting to the temperate zones. Fertility restorers and CMS were introduced for F-1 hybrid breeding. These phenotypes are often recessive, so information on the alleles responsible would be helpful for sorghum breeding. We expect these DNA markers to facilitate and improve the efficiency of F-1 sorghum breeding programs.