Tailoring Cellular Uptake of Conjugated Polymer Nanoparticles Using Modular Amphiphilic Peptide Capping Ligands
Tailoring Cellular Uptake of Conjugated Polymer Nanoparticles Using Modular Amphiphilic Peptide Capping Ligands
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DOI：
10.1021/acs.chemmater.5b03337
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发表时间：
2015-10-13
影响因子：
8.6
通讯作者：
Stevens, Molly M.
中科院分区：
文献类型：
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作者：
Almeida, Carina S.;Herrmann, Inge K.;Stevens, Molly M.
共轭聚合物具有极高的亮度、消光系数和光稳定性，作为生物医学应用的荧光探针具有优异的品质。以纳米颗粒形式封装这些疏水性聚合物可以转移到水性环境中并构建高性能荧光纳米颗粒结构，并且迄今为止已经证明了几种表面覆盖策略。在这里，我们描述了基于定制设计的两亲性肽的共轭聚合物纳米颗粒的新型多功能封端配体的开发。这些多功能肽配体提供保护性亲水帽层、用于进一步缀合的化学手柄，以及通过改变特定氨基酸序列来调节的定向生物活性。我们表明（i）细胞摄取可以作为肽组成的函数进行调节，并且（ii）纳米颗粒在使用的条件下没有显示出毒性迹象，这是开发这些临床使用技术时的一个重要的健康和环境问题。最后，我们证明这种一锅法可以应用于三类共轭聚合物，并展示多色成像的潜力。
Conjugated polymers possess excellent qualities as fluorescent probes for biomedical applications, because of their extremely high brightness, extinction coefficients, and photostability. Encapsulating these hydrophobic polymers in nanoparticulate form allows transfer to aqueous environments and construction of high-performance fluorescent nanoparticle constructs, and several surface capping strategies have been demonstrated to date. Here, we describe the development of a new class of multifunctional capping ligands for conjugated polymer nanoparticles based on custom-designed amphiphilic peptides. These versatile peptide ligands provide a protective hydrophilic capping layer, chemical handles for further conjugation, and directed biological activity tuned by altering the specific amino acid sequence. We show that (i) cellular uptake can be regulated as a function of peptide composition, and (ii) the nanoparticles show no signs of toxicity under the conditions used, which is a vital health and environmental issue when developing these technologies for clinical use. Finally, we demonstrate that this one-pot method can be applied can be applied to three classes of conjugated polymers and demonstrate potential for multicolor imaging.