The Preparation and Some Properties of Fibrinogen Precipitated from Human Plasma by Glycine.∗
The Preparation and Some Properties of Fibrinogen Precipitated from Human Plasma by Glycine.∗
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甘氨酸从人血浆中沉淀纤维蛋白原的制备及一些性质*
DOI：
10.3181/00379727-113-28553
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发表时间：
1963
影响因子：
--
通讯作者：
L. Tocantins
中科院分区：
文献类型：
--
作者：
L. A. Kazal;S. Amsel;O. Miller;L. Tocantins
摘要 描述了一种制备凝血酶可凝蛋白前98%、冻干后可凝蛋白94%的纤维蛋白原的方法。用0.78饱和度的甘氨酸从柠檬酸化的无凝血酶原血浆中沉淀纤维蛋白原。 pH 6.1–6.3，20°C，并通过甘氨酸再沉淀纯化。生物微量污染物为纤溶酶原、纤维蛋白稳定因子和抗血友病球蛋白；未发现凝血酶原和纤溶酶。尽管按照可凝固性标准，用甘氨酸沉淀法制备的纤维蛋白原非常纯，并且其性质与其他纤维蛋白原制剂相似，但移动边界电泳和超速离心却揭示了两种不同比例的组分。当通过用甘氨酸完全饱和血浆来制备纤维蛋白原时，β球蛋白（可能是冷不溶性球蛋白）是主要污染物。血浆中纤维蛋白原在 0.75 至 2.0 m 甘氨酸之间的沉淀遵循 Cohn 对数溶解度关系；盐析常数(Ks)为0.68。
Summary A method was described for the preparation of fibrinogen with 98% thrombin clottable protein before, and 94% clottable protein after lyophilization. The fibrinogen was precipitated from citrated prothrombinfree plasma with glycine at 0.78 saturation. pH 6.1–6.3, 20°C and purified by reprecipitation with glycine. The biological trace contaminants were plasminogen, fibrin stabilizing factor and antihemophilic globulin; prothrombin and plasmin were not found. Although fibrinogen prepared by the glycine precipitation method was very pure by the criterion of clottability and its properties were similar to those of other fibrinogen preparations, moving boundary electrophoresis and ultracentrifugation nevertheless disclosed two components in different proportions. When fibrinogen was prepared by fully saturating plasma with glycine, a beta globulin, presumably cold insoluble globulin, was a major contaminant. The precipitation of fibrinogen from plasma between 0.75 and 2.0 m glycine followed the logarithmic solubility relationship of Cohn; the salting-out constant (Ks) was 0.68.