Colorimetric immunoassay for rapid detection of Vibrio parahaemolyticus
Colorimetric immunoassay for rapid detection of Vibrio parahaemolyticus
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比色免疫分析法快速检测副溶血弧菌
DOI：
10.1007/s00604-017-2523-6
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发表时间：
2017-12-01
影响因子：
5.7
通讯作者：
Li, Juan
中科院分区：
文献类型：
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作者：
Liu, Yushen;Zhao, Chao;Li, Juan
副溶血性弧菌（V.副溶血性弧菌）是最常见的食源性病原体之一。作者描述了一种副溶血性弧菌的快速比色测定方法，该方法基于基于磁珠的夹心免疫测定和通过酶模拟物的信号放大的组合。二氧化锰纳米颗粒被用作人工氧化酶，在氧气存在下氧化3,3',5,5'-四甲基联苯胺，形成蓝色（易于观察）的产物，其最大吸收波长为652nm。通过结合磁珠优越的捕获效率和酶模拟物的高催化活性，该方法可以检测10至10(5)cfu范围内的副溶血性弧菌浓度。无预富集，检测限低至10 cfu.mL（-1）。加标牡蛎样品的回收率为87.5% ~ 106.0%。该检测方法快速、灵敏、特异、专属性强。在我们看来，它在副溶血性弧菌的快速仪器和现场视觉检测中显示出前景。
Vibrio parahaemolyticus (V. parahaemolyticus) is one of the most common food-borne pathogens. The authors describe a rapid colorimetric assay for V. parahaemolyticus that is based on a combination of a magnetic bead-based sandwich immunoassay and signal amplification via an enzmye mimic. MnO2 nanoparticles are used as an artificial oxidase that oxidizes 3,3', 5,5'-tetramethylbenzidine in the presence of oxygen to form a blue (and readily visible) product with an absorption maximum at 652 nm. By combining the superior capture efficiency ofmagnetic beads with the high catalytic activity of the enzmye mimic, this method can detect V. parahaemolyticus concentration in the range between 10 to 10(5) cfu.mL(-1) without pre-enrichment, and the limit of detection is as low as 10 cfu.mL(-1). Recoveries ranging from 87.5% to 106.0% are found when analyzing spiked oyster samples. The assay is rapid, sensitive, and specific and specific. In our perception, it shows promise in rapid instrumental and on-site visual detection of V. parahaemolyticus.