Domoic acid production in Nitzschia sp (Bacillariophyceae) isolated from a shrimp-culture pond in Do Son, Vietnam
Domoic acid production in Nitzschia sp (Bacillariophyceae) isolated from a shrimp-culture pond in Do Son, Vietnam
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DOI：
10.1046/j.1529-8817.2000.99209.x
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发表时间：
2000-12-01
影响因子：
2.9
通讯作者：
Kodama, M
中科院分区：
文献类型：
--
作者：
Kotaki, Y;Koike, K;Kodama, M
在新发现的海洋菱形藻（Nitzschia navis-varingica Lundholm et Moestrup）的分批培养中检测到神经兴奋性氨基酸软骨藻酸（DA）。DA的产量（1.7 pg细胞（-1））在多系拟菱形藻（Hasle）Hasle报告的水平范围内。DA的产量在指数生长期后期开始，在稳定生长期早期达到最大值。无菌培养物中的最大DA水平降低到非无菌培养物的一半左右（0.9 pg细胞（-1）vs. 1.7 pg细胞（-1）），表明新物种的DA生产受到细菌的影响。
Domoic acid (DA), a neuroexcitatory amino acid, was detected in batch culture of the newly recognized species Nitzschia navis-varingica Lundholm et Moestrup, The production of DA by this diatom was confirmed by electrospray ionization mass spectrometry, The diatom was collected from a shrimp-culture pond in Do Son, Vietnam, The production of DA (1.7 pg cell(-1)) is within the levels reported for Pseudo-nitzchia multiseries (Hasle) Hasle, The DA production started during the late exponential growth phase and reached a maximum during the early stationary growth phase. Maximum DA levels in the axenic culture decreased to about half that of the nonaxenic culture (0.9 pg cell(-1) vs. 1.7 pg cell(-1)), suggesting that DA production by the new species is influenced by bacteria.