The Modified High-Density Survival Assay is the Useful Tool to Predict the Effectiveness of Fractionated Radiation Exposure
The Modified High-Density Survival Assay is the Useful Tool to Predict the Effectiveness of Fractionated Radiation Exposure
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DOI：
10.1269/jrr.09094
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发表时间：
2010-05-01
影响因子：
2
通讯作者：
Fukumoto, Manabu
中科院分区：
文献类型：
--
作者：
Kuwahara, Yoshikazu;Mori, Miyuki;Fukumoto, Manabu
高密度存活（HDS）试验最初是为了评估癌细胞在细胞间通讯的影响下对治疗药物的反应。我们简化了原始HDS测定法，并研究了其用于检测细胞辐射抗性的适用性。我们最近定义了临床相关的放射抗性（CRR）细胞。其在体外每天暴露于2戈瑞（戈伊）的X射线超过30天后继续增殖。我们建立了人CRR细胞系，分别是来自HepG 2的HepG 2 -8960-R和来自SAS的SAS-R1和-R2。为了尝试应用HDS测定来检测具有临床相关性的辐射抗性，我们通过对暴露于X射线后存活细胞的总数进行评分来简化原始HDS测定。改良HDS试验成功检测出具有临床相关性的放射抗性。改良HIM法检测CRR表型。因此，我们认为本研究中提出的改良HDS测定法是预测分割放射治疗对恶性肿瘤的有效性的有力工具。
The high-density survival (HDS) assay was originally elaborated to assess cancer cell responses to therapeutic agents under the influence of intercellular communication Here. we simplified the original HDS assay and studied its applicability for the detection of cellular radioresistance. We have recently defined clinically relevant radioresistant (CRR) cells. which continue to proliferate with daily exposure to 2 gray (Gy) of X-rays for more than 30 clays in vitro We established human CRR cell lines, HepG2-8960-R from HepG2, and SAS-R1 and -R2 from SAS, respectively In an attempt to apply the HDS assay to detect radioresistance with clinical relevance, we simplified the original HDS assay by scoring the total number of surviving cells after exposure to X-rays. The modified HDS assay successfully detected radioresistance with clinical relevance. The modified HIM assay detected CRR phenotype. which is not always detectable by clonogenic assay Therefore, we believe that the modified HDS assay presented in this study is a powerful tool to predict the effectiveness of fractionated radiotherapy against malignant tumors.