A quantitative comparison of mechanoelectrical transduction in vestibular and auditory hair cells of neonatal mice
A quantitative comparison of mechanoelectrical transduction in vestibular and auditory hair cells of neonatal mice
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DOI：
10.1098/rspb.1997.0087
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发表时间：
1997-04-22
影响因子：
4.7
通讯作者：
Kros, CJ
中科院分区：
文献类型：
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作者：
Geleoc, GSG;Lennan, GWT;Kros, CJ
用液体射流刺激新生小鼠的前庭毛细胞（vhc）和耳蜗外毛细胞（OHCs）。研究了射流力、束位移和传感器电流之间的关系。vhc中换能器平均最大电导为2.6 nS，约为OHCs （5.5 nS）的一半，最大记录值分别为4.1 nS和9.2 nS。在一些OHCs中观察到单个112 pS换能器通道的活性，从而可以估计通道的最大数量：在vhc中多达36个，在OHCs中多达82个，相当于每个尖端链接约有一个换能器通道。VHC束需要约330 nm的尖端位移来激活90%的最大换能器电导，而OHC束则需要150 nm。由于VHC束的长度更大，这对应于两种轴的2度旋转。VHC束的平动刚度为OHCs的1 / 7。转换为旋转刚度几乎消除了这种差异。毛束的旋转而不是平移决定了换能器通道的门控，与毛束的高度或细胞的起源无关。
Vestibular hair cells (VHCs) and cochlear outer hair cells (OHCs) of neonatal mice were stimulated by a fluid jet directed at their stereociliary bundles. Relations between the force exerted by the jet, bundle displacement, and the resulting transducer current were studied. The mean maximum transducer conductance in VHCs (2.6 nS) was about half that of the OHCs (5.5 nS), with the largest recorded values being 4.1 nS and 9.2 nS, respectively. In some OHCs activity of a single, 112 pS transducer channel was observed, allowing an estimate of the maximum number of channels: up to 36 in VHCs and 82 in OHCs, corresponding to about one transducer channel per tip link. The VHC bundles required about 330 nm of tip displacement to activate 90% of the maximum transducer conductance, compared to 150 nm for the OHC bundles. This corresponded to 2 deg of rotation about their pivots for both, due to the greater length of the VHC bundles. The VHC bundles' translational stiffness was one-seventh of that of the OHCs. Conversion to rotational stiffness almost abolished this difference. Rotation of the hair bundle rather than translation determines the gating of the transducer channels, independent of bundle height or origin of the cells.