Follicle-stimulating hormone receptor (FSHR) in Chinese alligator, Alligator sinensis: Molecular characterization, tissue distribution and mRNA expression changes during the female reproductive cycle
Follicle-stimulating hormone receptor (FSHR) in Chinese alligator, Alligator sinensis: Molecular characterization, tissue distribution and mRNA expression changes during the female reproductive cycle
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扬子鳄卵泡刺激素受体（FSHR）：雌性生殖周期中的分子特征、组织分布和 mRNA 表达变化
DOI：
10.1016/j.anireprosci.2015.02.008
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发表时间：
2015-05-01
影响因子：
2.2
通讯作者：
Wu, Xiaobing
中科院分区：
文献类型：
--
作者：
Zhang, Rui;Zhang, Shengzhou;Wu, Xiaobing
促卵泡激素（FSH）在脊椎动物的繁殖中发挥着核心作用，FSH 的作用是由卵巢颗粒细胞上的 FSH 受体（FSHR）介导的。本研究报告了扬子鳄FSHR（caFSHR）的克隆和特性，及其在繁殖周期中的组织分布和mRNA表达变化。 caFSHR的成熟蛋白表现出糖蛋白激素受体家族的典型特征，但与其他脊椎动物FSHR相比也存在一些显着差异。推导的caFSHR氨基酸序列与中华鳖的同源性为85%，与鸟类的同源性为84-87%，与哺乳动物的同源性为77-78%，与两栖动物的同源性为67-73%，与鱼类的同源性为51-58%。 FSHR 氨基酸序列的系统发育树分析表明，短吻鳄聚集到鸟分支中。组织表达分析显示，caFSHR不仅在卵巢中表达，在胃、肠、胰腺、肝脏和输卵管中也有相似水平的表达，而在心脏、胸腺或甲状腺中未检测到。 caFSHR 在卵巢中的表达在 5 月（繁殖前期）较高，在繁殖期的 7 月达到峰值，并在整个 9 月（繁殖后期）保持高水平。 11 月（冬眠期）表达显着下降，然后 1 月至 3 月（冬眠期）保持相对较低水平。 FSHR表达的这些时间变化表明它在扬子鳄雌性生殖周期中促进卵巢发育发挥重要作用。 (C) 2015 Elsevier B.V. 保留所有权利。
The follicle-stimulating hormone (FSH) plays a central role in vertebrate reproduction, with the actions of FSH mediated by FSH receptors (FSHRs) on the granulosa cells of the ovary. The present study reports the cloning and characterization of FSHR in Chinese alligator, Alligator sinensis (caFSHR), and its tissue distribution and mRNA expression changes during the reproductive cycle. The mature protein of caFSHR displays typical features of the glycoprotein hormone receptor family, but also contains some remarkable differences when compared with other vertebrate FSHRs. The deduced amino acid sequence of the caFSHR shares identity of 85% with Chinese softshell turtle, 84-87% with birds, 77-78% with mammals, 67-73% with amphibians and 51-58% with fishes. Phylogenetic tree analysis of the FSHR amino acid sequence indicated that alligators cluster into the bird branch. Tissue expression analysis showed that caFSHR was not only expressed in the ovary, but also in the stomach, intestine, pancreas liver and oviduct at similar levels, while it was not detectable in heart, thymus or thyroid. Expression of caFSHR in the ovary is high in May (breeding prophase) and peaks in July during the breeding period, where it is maintained at high levels through September (breeding anaphase). Expression decreases significantly in November (hibernating period) and then remains relatively low from January to March (hibernating period). These temporal changes in FSHR expression suggest that it plays an important role in promoting ovarian development during the female reproductive cycle of Chinese alligator. (C) 2015 Elsevier B.V. All rights reserved.