CELL-LINES ESTABLISHED BY A TEMPERATURE-SENSITIVE SIMIAN VIRUS-40 LARGE-T-ANTIGEN GENE ARE GROWTH RESTRICTED AT THE NONPERMISSIVE TEMPERATURE
CELL-LINES ESTABLISHED BY A TEMPERATURE-SENSITIVE SIMIAN VIRUS-40 LARGE-T-ANTIGEN GENE ARE GROWTH RESTRICTED AT THE NONPERMISSIVE TEMPERATURE
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DOI：
10.1128/mcb.9.4.1672
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发表时间：
1989-04-01
影响因子：
5.3
通讯作者：
SHARP, PA
中科院分区：
文献类型：
--
作者：
JAT, PS;SHARP, PA
用猴病毒40早期区突变体tsA58编码的耐热性大T抗原，建立了大鼠胚胎成纤维细胞克隆细胞系。这些细胞系在允许温度下连续生长，但当转移到非允许温度时，生长迅速停止。生长停滞发生在细胞周期的G1或G2期。在生长停止后，通过一般蛋白质合成和排除台盼蓝的能力来检测细胞的代谢活性。这些细胞系在不允许的温度下分裂的能力不容易被外源引入的其他核癌基因所补充。这一发现表明，要么这些基因通过不同的途径建立细胞，要么一个致癌基因的永生化导致细胞状态的精细平衡，而另一个建立基因无法充分补充这种状态。
The thermolabile large T antigen, encoded by the simian virus 40 early-region mutant tsA58, was used to establish clonal cell lines derived from rat embryo fibroblasts. These cell lines grew continuously at the permissive temperature but upon shift-up to the nonpermissive temperature showed rapiply arrested growth. The growth arrest occurred in either the G1 or G2 phase of the cell cycle. After growth arrest, the cells remained metabolically active as assayed by general protein synthesis and the ability to exclude trypan blue. The inability of these cell lines to divide at the nonpermissive temperature was not readily complemented by the exogenous introduction of other nuclear oncogenes. This finding suggests that either these genes establish cells via different pathways or that immortalization by one oncogene results in a finely balanced cellular state which cannot be adequately complemented by another establishment gene.