PROLIFERATION AND DIFFERENTIATION PROPERTIES OF BIPOTENT GLIAL PROGENITOR-CELL LINES IMMORTALIZED WITH THE ADENOVIRUS E1A GENE
PROLIFERATION AND DIFFERENTIATION PROPERTIES OF BIPOTENT GLIAL PROGENITOR-CELL LINES IMMORTALIZED WITH THE ADENOVIRUS E1A GENE
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DOI：
10.1002/jnr.490360204
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发表时间：
1993-10-01
影响因子：
4.2
通讯作者：
EVRARD, C
中科院分区：
文献类型：
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作者：
GALIANA, E;BERNARD, R;EVRARD, C
通过将腺病毒EIA基因转移到原代培养的大鼠脑细胞中，可以使双能性胶质祖细胞永生化。获得的细胞系在表型上未转化，保持生长接触抑制，并且能够分化，除非它们被转化癌基因转染。根据生长条件，这些永生化细胞表达不同的少突胶质细胞或星形胶质细胞特异性标记物和基因。在无血清培养基中接种后，显示A2B5单克隆抗体识别的神经节苷类，然后依次表达04个表位、半乳糖脑苷、髓磷脂蛋白DM20。在血清补充培养基中培养时，细胞先表达A2B5表位，然后短暂表达04和半乳糖脑苷；到达汇合点后，细胞开始共表达胶质原纤维酸性蛋白和谷氨酰胺合成酶。这些结果表明，细胞系可以经历类似于O-2A祖细胞和具有塑性过程的胶质亚群的分化。这些细胞也通过稳定导入nlslacZ报告基因进行遗传标记。因此，这些细胞系可用于研究体外直接相互作用，或用于移植后的研究。它们也应该为研究参与承诺和控制这种细胞系的增殖和分化的机制提供一个模型。这一建议是一致的数据表明，一个新的基因编码蛋白质的螺旋-环-螺旋结构域的生长停滞依赖的差异表达。(C) 1993 Wiley-Liss, Inc。
Bipotent glial progenitors have been immortalized by the transfer of the adenovirus EIA gene into primary cultured cells from embryonic rat brain. The lines obtained are phenotypically untransformed, retain growth contact-inhibition, and are able to differentiate, unless they are surtransfected with transforming oncogenes. Depending on the growth conditions, these immortalized cells express differentially either oligodendrocyte or astrocyte-specific markers and genes. After being seeded in serum-free medium, they display gangliosides recognized by A2B5 monoclonal antibody, and then they express sequentially 04 epitopes, galactocerebroside, and the myelin protein DM20. When grown in serum-supplemented medium, the cells express at first A2B5 epitopes, and then transiently 04 and galactocerebroside; after reaching confluence, 04 and galactocerebroside become undetectable, whereas the cells begin to coexpress glial fibrillary acidic protein and glutamine synthetase. These results indicate that the cell lines can undergo a differentiation reminiscent both of O-2A progenitors and of plastic process-bearing glial sub-populations. The cells were also genetically marked by the stable introduction of the nlslacZ reporter gene. Thus, the lines could be useful for studying direct interactions in vitro, or for post-grafting investigations. They should also provide a model for studying the mechanisms involved in the commitment and in the control of proliferation and differentiation of this cell lineage. This suggestion is consistent with the data indicating a growth arrest-dependent differential expression of a novel gene encoding a protein with a helix-loop-helix domain. (C) 1993 Wiley-Liss, Inc.