Rapid detection of different DNA analytes using a single electrochemical sensor
Rapid detection of different DNA analytes using a single electrochemical sensor
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DOI：
10.1016/j.snb.2019.04.149
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发表时间：
2019-08-15
影响因子：
8.4
通讯作者：
Chumbimuni-Torres, Karin Y.
中科院分区：
文献类型：
--
作者：
Mills, Dawn M.;Foguel, Marcos V.;Chumbimuni-Torres, Karin Y.
开发了一种利用单个电化学传感器检测两种不同DNA序列的独特平台。该传感器由固定在固体表面的通用杆环探头和两条分析物特定的适配器链组成。这些适配子链与核酸分析物杂交，提供高度特异的识别和高结合亲和力。用尿素和水简单快速地冲洗就可以再生万能探头。作为概念的证明，我们区分了寨卡病毒的长靶序列(141nt.)和登革热(114nt.)含有二级结构的病毒。所提出的传感器提供了使用单个电化学传感器快速且经济高效地检测潜在的任何DNA或RNA序列的平台。
A unique platform for the detection of two distinct DNA sequences by using one single electrochemical sensor was developed. The sensor consists of a universal stem loop probe attached to a solid surface, and two analyte-specific adaptor strands. These adaptor strands hybridize to a nucleic acid analyte and provide both highly specific recognition and high binding affinity. The universal probe can be regenerated by a simple and quick rinse with urea and water. As a proof of concept, we differentiated long target sequences of Zika (141 nt.) and Dengue (114 nt.) viruses that contain secondary structures. The proposed sensor provides a platform for rapid and cost-efficient detection of potentially any DNA or RNA sequence using a single electrochemical sensor.