Age and gender effects on microglia and astrocyte numbers in brains of mice
Age and gender effects on microglia and astrocyte numbers in brains of mice
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DOI：
10.1016/s0006-8993(02)03475-3
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发表时间：
2002-11-22
期刊：
影响因子：
2.9
通讯作者：
Ingram, DK
中科院分区：
文献类型：
--
作者：
Mouton, PR;Long, JM;Ingram, DK
正常大脑衰老过程中发生的形态学变化尚不清楚。本研究采用现代体视学方法研究了年龄和性别对C57B1/6NNIA（136）小鼠海马结构中星形胶质细胞和小胶质细胞总数的影响。利用免疫细胞化学分别观察星形胶质细胞和小胶质细胞的胶质纤维酸性蛋白（GFAP）和补体受体3 (Mac-1)，并利用光学分划法估计齿状回（DG）和CA1区每种细胞类型的数量。结果显示，与136只年轻雌性小鼠相比，老年雌性小鼠的小胶质细胞和星形胶质细胞数量显著增加（接近20%）。我们还报道，雌性B6小鼠在DG和CA1区域的星形胶质细胞和小胶质细胞平均比同龄雄性C57B1/6J小鼠多25-40%。由于星形胶质细胞和小胶质细胞被认为是性激素的目标，性激素和生殖衰老的影响可能是这些发现的原因。(C) 2002 Elsevier Science B.V.版权所有
The morphological changes that occur during normal brain aging are not well understood. This study used modem stereology to assess the effects of age and gender on total numbers of astrocytes and microglia in the hippocampal formation in C57B1/6NNIA (136) mice. Astrocytes and microglia were visualized using immunocytochemistry for glial fibrillary acidic protein (GFAP) and complement receptor 3 (Mac-1), respectively, and numbers of each cell type in dentate gyrus (DG) and CA1 regions were estimated using the optical fractionator method. The results reveal significantly greater (similar to20%) numbers of microglia and astrocytes in aged females compared to young female 136 mice. We also report that on average female B6 mice have 25-40% more astrocytes and microglia in DG and CA1 regions than age-matched male C57B1/6J mice. Since astrocytes and microglia are thought to be targets of gonadal hormones, the effects of sex hormones and reproductive aging may be responsible for these findings. (C) 2002 Elsevier Science B.V. All rights reserved.