Spatiotemporal dynamics of the tomato fruit transcriptome under prolonged water stress
Spatiotemporal dynamics of the tomato fruit transcriptome under prolonged water stress
复制标题
长期水分胁迫下番茄果实转录组的时空动态
DOI：
10.1093/plphys/kiac445
复制
发表时间：
2022
期刊：
影响因子：
7.4
通讯作者：
Vrebalov, Julia
中科院分区：
文献类型：
--
作者：
Nicolas, Philippe;Shinozaki, Yoshihito;Powell, Adrian;Philippe, Glenn;Snyder, Stephen I.;Bao, Kan;Zheng, Yi;Xu, Yimin;Courtney, Lance;Vrebalov, Julia
水分供应影响植物生长和发育的方方面面；然而，大多数关于植物对干旱的反应的研究集中在营养器官，特别是根和叶。水果是具有不同组织类型的复杂器官，人们对水果干旱适应反应的分子基础知之甚少。为了更全面地了解干旱条件下控制果实发育的分子机制，我们对番茄(Solanum Lycopsicum)的一系列果实组织的转录谱进行了分析，这些组织跨越了番茄的早期生长到成熟，并从不同强度的水分胁迫下生长的植物中收集了这些组织的转录。此外，我们比较了果实和叶片中的转录变化，以突出营养器官和生殖器官中不同和保守的转录组特征。我们在不同的果实组织和叶片中观察到广泛和多样化的基因重新编程，每个组织和叶片都与对干旱适应的独特反应有关。这些变化包括发育中的果实胎盘和成熟果实种子中的主要转录变化，分别与细胞生长和表观遗传调节有关。代谢和激素途径的变化，如与淀粉、类胡萝卜素、茉莉酸和乙烯代谢有关的途径，与不同的果实组织和发育阶段有关。基因共表达网络分析为组织特异性调控对水分胁迫的不同反应提供了进一步的见解。我们的数据突出了番茄果实干旱反应的时空特异性，并表明了在营养和生殖发育阶段参与干旱驯化的已知和未揭示的分子调控机制。
Water availability influences all aspects of plant growth and development; however, most studies of plant responses to drought have focused on vegetative organs, notably roots and leaves. Far less is known about the molecular bases of drought acclimation responses in fruits, which are complex organs with distinct tissue types. To obtain a more comprehensive picture of the molecular mechanisms governing fruit development under drought, we profiled the transcriptomes of a spectrum of fruit tissues from tomato (Solanum lycopersicum), spanning early growth through ripening and collected from plants grown under varying intensities of water stress. In addition, we compared transcriptional changes in fruit with those in leaves to highlight different and conserved transcriptome signatures in vegetative and reproductive organs. We observed extensive and diverse genetic reprogramming in different fruit tissues and leaves, each associated with a unique response to drought acclimation. These included major transcriptional shifts in the placenta of growing fruit and in the seeds of ripe fruit related to cell growth and epigenetic regulation, respectively. Changes in metabolic and hormonal pathways, such as those related to starch, carotenoids, jasmonic acid, and ethylene metabolism, were associated with distinct fruit tissues and developmental stages. Gene coexpression network analysis provided further insights into the tissue-specific regulation of distinct responses to water stress. Our data highlight the spatiotemporal specificity of drought responses in tomato fruit and indicate known and unrevealed molecular regulatory mechanisms involved in drought acclimation, during both vegetative and reproductive stages of development.