Cells from Hertwig's epithelial root sheath do not transcribe amelogenin.
Cells from Hertwig's epithelial root sheath do not transcribe amelogenin.
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赫特维希上皮根鞘细胞不转录牙釉蛋白。
DOI：
10.1111/j.1600-0765.1991.tb01624.x
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发表时间：
1991
影响因子：
3.5
通讯作者：
Snead,ML
中科院分区：
文献类型：
--
作者：
Luo,W;Slavkin,HC;Snead,ML
最近的实验证据已经导致了这样的解释，即“釉质样”物质通过Hertwig上皮根鞘细胞（HERS细胞）沿着形成中的小鼠磨牙根表面沉积沿着，并且这种物质是牙骨质形成发育程序的组成部分。在这项研究中描述的实验策略是检查选择的发育阶段的根形成的小鼠第一和第二下颌磨牙，以本地化的细胞位点的amelogeningene转录本使用高分辨率原位杂交。釉原蛋白是牙釉质的主要结构蛋白，在哺乳动物中在DNA和氨基酸水平上高度保守。在原位杂交的灵敏度范围内，我们不能利用cRNA或寡脱氧核苷酸探针在与小鼠磨牙牙根形成相关的特定发育阶段的HERS细胞内定位釉原内转录物。相比之下，先前使用抗肽抗体的研究提供了HERS细胞衍生产物中釉原蛋白结构域的免疫组织化学定位。对于这些HERS细胞衍生的蛋白含有两个釉原蛋白表位，但不能产生核酸杂交信号表明，基因重排和/或来自结构基因座的信使RNA的替代加工操作以产生在核苷酸水平上共享不足互补性的免疫相关基序，以允许通过杂交进行有效检测。据推测，HERS细胞合成的蛋白质含有釉原蛋白结构域，这些蛋白质参与牙骨质形成。然而，这些釉质相关蛋白既不相同，也不与冠状面典型的釉原内转录物共线。
Recent experimental evidence has led to the Interpretation that “enamel‐like” material is deposited along the forming mouse molar root surface by cells of Hertwig's epithelial root sheath (HERS cells) and that this material is integral to the developmental program for cementogenesis. The experimental strategy described in this study was to examine selected developmental stages of root formation for mouse first and second mandibular molars in order to localize the cellular sites ofamelogeningene transcripts using high resolutionin situhybridization. Amelogenin is the major structural protein of coronal enamel and is highly conserved among mammalian species at the DNA and amino acid sequence level. Within the limits of sensitivity forin situhybridization and utilizing either cRNAs or oligodeoxynucleotide probes, we were unable to localizeamelogenintranscripts within HERS cells from selected developmental stages associated with mouse molar root formation. In contrast, previous studies using antipeptide antibodies have provided immuno‐histochemical localization of amelogenin domains in HERS cell‐derived products. For these HERS cell‐derived proteins to contain both amelogenin epitopes and yet fail to yield nucleic acid hybridization Signals suggests that either gene rearrangement and/or alternative processing of messenger RNAs from the structural gene locus operate to produce immunologically related motifs sharing insufficient complementarity at the nucleotide level to permit efficient detection by hybridization. It is postulated that HERS cells synthesize proteins which contain amelogenin domains and that these proteins participate during cementogenesis. However, these enamel‐related proteins are neither identical to, nor collinear with coronal canonicalamelogenintranscripts.