High polymorphism and resolution in targeted fingerprinting with combined ß-tubulin introns
High polymorphism and resolution in targeted fingerprinting with combined ß-tubulin introns
复制标题
DOI：
10.1007/s11032-007-9087-9
复制
发表时间：
2007-10-01
影响因子：
3.1
通讯作者：
Giani, Silvia
中科院分区：
文献类型：
--
作者：
Breviario, Diego;Baird, Wm. Vance;Giani, Silvia
微管蛋白多态(TBP)最初是一种分析植物遗传多样性的新方法。TBP是基于通过聚合酶链式反应扩增β-微管蛋白基因家族编码区第一内含子而产生的多态。虽然该方法在一些植物种和品种的遗传评价中取得了成功，但由于β-微管蛋白基因家族第一内含子变异有限，分子标记数量较少。我们现在已经通过引入β-微管蛋白基因的第二内含子作为有价值的分子标记来源来纠正这一限制。我们表明，两个内含子的结合使用大大增加了分子标记的数量，并导致了对物种/品种关系的可靠评估。在对Brassica进行初步验证后，该组合方法在Eleusine和Arachim种上进行了测试。对于这两种物种，都得到了可靠的物种关系评估，这与最近发表的研究结果一致，这些研究是通过包括DNA测序在内的更详细的方法得出的。组合TBP是一种可靠、重复性好、简单、快速、易于评分的方法，对育种计划和物种和品种评估非常有用。
Tubulin-Based-Polymorphism (TBP) was originally introduced as a novel method for assaying genetic diversity in plants. TBP is based on polymorphism resulting from the PCR-mediated amplification of the first intron in the coding region of the beta-tubulin gene family. Although, the method was successful in genetic assessment of some plant species and varieties, it suffered from low number of molecular markers due to limited variation in the first intron of beta-tubulin gene family. We have now rectified this limitation by introducing the second intron of the beta-tubulin genes as a valuable source of molecular markers. We show that the combined use of the two introns substantially increases the number of molecular markers and results in a reliable assessment of species/varieties relationships. After a preliminary validation on Brassica, this new combinatorial method was tested on species of Eleusine and Arachis. For both, reliable assessment of species relationships were obtained that were consistent with recently published studies resulting from more elaborated methods including DNA sequencing. Combinatorial TBP is a reliable, reproducible, simple, fast, and easy to score method that is very useful for breeding programs and species and variety assessments.