BACTERICIDAL AND WOUND-HEALING PROPERTIES OF SODIUM HYPOCHLORITE SOLUTIONS THE 1991 LINDBERG AWARD
BACTERICIDAL AND WOUND-HEALING PROPERTIES OF SODIUM HYPOCHLORITE SOLUTIONS THE 1991 LINDBERG AWARD
复制标题
DOI：
10.1097/00004630-199109000-00005
复制
发表时间：
1991-01-01
期刊：
影响因子：
--
通讯作者：
ROBSON M C
中科院分区：
文献类型：
--
作者：
HEGGERS J P;SAZY J A;ROBSON M C
次氯酸钠对伤口愈合元素的毒性作用一直局限于次氯酸钠浓度的有限范围内。我们研究了次氯酸钠浓度在不同时间间隔内的抗菌活性和组织毒性。我们试图找到既杀微生物又无毒的有效治疗浓度。革兰氏阴性和革兰氏阳性分离株（0.1/ml）。108/ml)加入不同浓度的缓冲和未缓冲次氯酸钠溶液中，以5分钟、10分钟、15分钟和30分钟为间隔测定杀菌活性。次氯酸钠浓度分别为0.25%、0.025%和0.0125%。同样浓度的成纤维细胞体外实验也在同样的时间间隔内进行了毒性测定。体内切口模型也被用来确定次氯酸钠治疗对伤口愈合的影响。在低至0.025%的浓度下观察到杀菌效果。在体内和体外，浓度为0.25%时均有组织毒性，0.025%时无组织毒性。虽然低于这个水平的浓度是无毒的，但它们没有杀菌作用。因此，浓度为0.025%的改良“Dakin’s”溶液作为液体敷料具有治疗效果，因为它保留了杀菌特性并消除了对伤口愈合的有害潜在影响。
Toxic effects of sodium hypochlorite on wound healing elements have been confined to a restricted range of sodium hypochlorite concentrations. We investigated concentrations of sodium hypochlorite for antibacterial activity and tissue toxicity at varying time intervals. We attempted to find the efficacious therapeutic concentration that was both microbicidal and nontoxic. Gram-negative and gram-positive isolates (0.1/ml of 1 .times. 108/ml) were introduced into various concentrations of buffered and unbuffered sodium hypochlorite solutions for determinations of bactericidal activity at 5-, 10-, 15-, and 30-minute intervals. Concentrations of sodium hypochlorite were 0.25%, 0.025%, and 0.0125%. In vitro assays with fibroblasts at the same concentrations were also performed to determine toxicity at the same time intervals. An in vivo incisional model was also used to determine the effects of sodium hypochlorite therapy on wound healing. Bactericidal effects were observed for concentrations as low as 0.025%. Tissue toxicity, both in vitro and in vivo, was observed at concentrations of 0.25% but not at a concentration of 0.025%. Although concentrations below this level were nontoxic, they were not bactericidal. Therefore a modified "Dakin's" solution at concentration of 0.025% is therapeutically efficacious as fluid dressing, since it preserves bactericidal properties and eliminates the detrimental potential on would healing.