The Application of Mitochondrial DNA Cytochrome Oxidase II Gene for the Identification of Forensically Important Blowflies in Western China
The Application of Mitochondrial DNA Cytochrome Oxidase II Gene for the Identification of Forensically Important Blowflies in Western China
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DOI：
10.1097/paf.0b013e31815b4889
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发表时间：
2007-12
期刊：
影响因子：
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通讯作者：
B. Ying;T. Liu;H. Fan;D. Wei;F. Wen;P. Bai;Jin Huang;Yingjian Hou
中科院分区：
文献类型：
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作者：
B. Ying;T. Liu;H. Fan;D. Wei;F. Wen;P. Bai;Jin Huang;Yingjian Hou
在人类尸体上发现的绿头苍蝇对估计死后时间和其他法医相关问题很重要。这些物种中的一些是难以区分形态，因此，分子鉴定方法进行了详细说明。在这里，我们描述了一种快速鉴定这些昆虫的分子方法。使用聚合酶链反应扩增特定的昆虫DNA片段，然后对扩增产物进行直接DNA测序。细胞色素氧化酶II序列的分析揭示了丰富的基因信息的核苷酸取代，可以确定丽蝇物种的物种组。与此相反，由于姐妹种的低水平的序列分歧，数据不能区分来自同一种组的分类群，即丝光绿蝇和绿蝇组内的物种。分子生物学数据支持丽蝇属内现有种组分类群的分离。由于目前核苷酸测序技术的速度和准确性，以及从mtDNA序列中获得的丰富的无定形取代，这种方法可以快速鉴定用于估计死后时间的物种。
Blowflies found on human corpses are important for the estimation of the postmortem interval and other questions of forensic relevance. Some of these species are difficult to differentiate morphologically, and therefore a molecular method was elaborated for species identification. Here, we describe a molecular method for rapid identification of these insects. Specific insect DNA fragments were amplified using the polymerase chain reaction, followed by direct DNA sequencing of the amplification products. Analysis of the cytochrome oxidase II sequences revealed abundant phylogenetically informative nucleotide substitutions that could identify blowfly species to species group. In contrast, because of the low level of sequence divergence of sister species, the data could not distinguish among taxa from the same species group, ie, the species within the Lucilia sericata and Lucilia cuprina groups. The molecular data support the existing species group separation of the taxa within the Calliphora. Because of the speed and accuracy of current nucleotide sequencing technology and the abundant apomorphic substitutions available from mtDNA sequences, this approach enables quick identification of species used for estimation of postmortem interval.