The effect of low-level laser therapy on the healing of hard palate mucosa and the oxidative stress status of rats
The effect of low-level laser therapy on the healing of hard palate mucosa and the oxidative stress status of rats
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DOI：
10.1111/jop.12106
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发表时间：
2014-02-01
影响因子：
3.3
通讯作者：
Uysal, Ersin
中科院分区：
文献类型：
--
作者：
Firat, Ela Tules;Dag, Ahmet;Uysal, Ersin
低强度激光治疗（LLLT）的生物刺激效应最近已经得到证实。本研究探讨了LLLT对大鼠腭粘骨膜伤口愈合和氧化应激状态的影响。材料和方法本研究中使用了42只体重250-300 g的雄性Wistar大鼠。使用直径为3 mm的活检打孔器在大鼠硬腭的粘骨膜中创建标准化全层伤口。术后开始使用波长为940 nm、剂量为10 J/cm 2的GaAlAs激光进行治疗，并在术后第2、4和6天重复治疗。在术后第7、14和21天处死每组7只动物。总抗氧化状态和总氧化状态测定serum.ResultsThe组织病理学结果显示，第7天的炎性细胞数量减少，增加有丝分裂活性的成纤维细胞的第14和第21天，和相同程度的胶原合成和血管化的第7天，14日，和21日在LLLT组与对照组相比。总氧化状态和总抗氧化状态之间没有显着差异，观察groups.ConclusionLLLT使用的GaAlAs激光在940 nm的波长和剂量为10 J/cm 2引起积极的愈合效果腭粘骨膜伤口可能通过诱导成纤维细胞。氧化应激状态不受LLLT的影响。
ObjectiveThe biostimulation effects of low‐level laser therapy (LLLT) have been demonstrated recently. This study investigated the effects of LLLT on palatal mucoperiosteal wound healing and oxidative stress status in rats.Material and methodForty‐two male Wistar rats weighing 250–300 g were used in this study. A standardized full‐thickness wound was created in the mucoperiosteum of the hard palates of the rats using a 3‐mm‐diameter biopsy punch. Treatment using a GaAlAs laser at a wavelength of 940 nm and a dose of 10 J/cm2was initiated after surgery and repeated on the 2nd, 4th, and 6th days post‐surgery. Seven animals from each group were sacrificed on the 7th, 14th, and 21st days after surgery. Total antioxidant status and total oxidative status were measured in serum.ResultsThe histopathological findings revealed reduced numbers of inflammatory cells on the 7th day, increased mitotic activity of fibroblasts on the 14th and 21st day, and the same degree of collagen synthesis and vascularization on the days 7, 14, and 21 in the LLLT group compared with the control group. No significant differences in total oxidative status and total antioxidant status were observed between the groups.ConclusionLLLT using a GaAlAs laser at a wavelength of 940 nm and a dose of 10 J/cm2elicited a positive healing effect on palatal mucoperiosteal wounds likely via the induction of fibroblasts. The oxidative stress status was not affected by LLLT.