Aminoacylation of short hairpin RNAs through kissing-loop interactions indicates evolutionary trend of RNA molecules
Aminoacylation of short hairpin RNAs through kissing-loop interactions indicates evolutionary trend of RNA molecules
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DOI：
10.1016/j.biosystems.2020.104206
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发表时间：
2020-11-01
期刊：
影响因子：
1.6
通讯作者：
Tamura, Koji
中科院分区：
文献类型：
--
作者：
Mutsuro-Aoki, Hiromi;Hamachi, Kokoro;Tamura, Koji
tRNA（Ala）受体茎中独特的G3：U 70碱基对已被证明是丙氨酰-tRNA合成酶（AlaRS）的关键识别位点。碱基对位于tRNA（微螺旋）L形结构的一个臂上，遗传密码可能是从原始的tRNA-aaRS（氨酰-tRNA合成酶）系统进化而来的。就tRNA的进化而言，在结构中引入G：U碱基对将是重要的。在这里，我们发现两个独立的短发夹RNA通过吻环相互作用改变它们的构象，最终形成一个迷你螺旋状结构，其中G3：U 70碱基对被纳入。该RNA系统可以被具有丙氨酰化活性的最小大肠杆菌AlaRS变体（AlaRS 442 N）适当地氨酰化。因此，通过吻环相互作用产生的特征性结构特征可能为RNA的进化提供重要线索。
The unique G3:U70 base pair in the acceptor stem of tRNA(Ala) has been shown to be a critical recognition site by alanyl-tRNA synthetase (AlaRS). The base pair resides on one of the arms of the L-shaped structure of tRNA (minihelix) and the genetic code has likely evolved from a primordial tRNA-aaRS (aminoacyl-tRNA synthetase) system. In terms of the evolution of tRNA, incorporation of a G:U base pair in the structure would be important. Here, we found that two independent short hairpin RNAs change their conformation through kissing-loop interactions, finally forming a minihelix-like structure, in which the G3:U70 base pair is incorporated. The RNA system can be properly aminoacylated by the minimal Escherichia coli AlaRS variant with alanylation activity (AlaRS442N). Thus, characteristic structural features produced via kissing-loop interactions may provide important clues into the evolution of RNA.