Multinucleated smooth muscles and mononucleated as well as multinucleated striated muscles develop during establishment of the male reproductive organs of Drosophila melanogaster.
Multinucleated smooth muscles and mononucleated as well as multinucleated striated muscles develop during establishment of the male reproductive organs of Drosophila melanogaster.
复制标题
DOI：
10.1016/j.ydbio.2012.07.022
复制
发表时间：
2012-10
影响因子：
2.7
通讯作者：
Loreen Susic-Jung;Christina Hornbruch-Freitag;Jessica Kuckwa;K. Rexer;U. Lammel;R. Renkawitz-Pohl
中科院分区：
文献类型：
--
作者：
Loreen Susic-Jung;Christina Hornbruch-Freitag;Jessica Kuckwa;K. Rexer;U. Lammel;R. Renkawitz-Pohl
黑腹龙的成年肌肉组织是在变态过程中形成的。我们对飞行和腿部肌肉组织了解甚多，但对男性生殖道周围的肌肉却知之甚少。雄性的内生殖器包括从性腺中长出来的睾丸；其余的生殖器官，如生殖腺（或附属腺）、射精管、精子泵和精囊，由生殖想像盘发育而来。我们分析了形成这些器官肌肉层的成肌细胞。在源自生殖器想象盘的成肌细胞中，转录因子DMef2的调控区域是活跃的。DMef2也是胚胎和成体成肌细胞分化和分化所必需的。我们可以区分出三种不同的肌肉类型：(i)睾丸周围类似脊椎动物平滑肌的多核肌肉；（ii）类似横纹肌的多核肌肉包括精囊和精子泵；（iii）包住射精管和射精管的单核横纹肌。参与胚胎肌发生的免疫球蛋白超家族成员，dumbated （Duf）和Sticks and Stones (Sns)，也在生殖器想象盘、肌肉分化期间的睾丸肌鞘和分泌性次级细胞中表达，这些细胞是包围肺原体的双核上皮的一部分。
The adult musculature in D. melanogaster forms during metamorphosis. Much is known about the flight and leg musculature, but not about the muscles surrounding the male reproductive tract. The inner genitalia of males consist of the testes, which emerge from the gonads; the remaining genital organs, i.e., paragonia (or accessory glands), ejaculatory duct, sperm pump, and seminal vesicles, develop out of the genital imaginal disc. We analyzed the myoblasts forming the muscle layers of these organs. In myoblasts derived from the genital imaginal disc, the regulatory region of the transcription factor DMef2 is active. DMef2 is also needed for specification and differentiation of embryonic and adult myoblasts. We could discriminate three different muscle types: (i) multinucleated muscles that resemble vertebrate smooth muscles surround the testes, (ii) multinucleated muscles that resemble striated muscles comprises seminal vesicles and the sperm pump, and (iii) mononucleated striated musculature encloses the paragonia and ejaculatory duct. Members of the immunoglobulin superfamily involved in embryonic myogenesis, Dumbfounded (Duf) and Sticks and Stones (Sns), were also expressed in the genital imaginal disc, in the muscle sheath of the testes during muscle differentiation and in the secretory secondary cells, which are part of the binucleated epithelia enclosing the paragonia.