Nanoparticle deposition onto biofilms.
Nanoparticle deposition onto biofilms.
复制标题
纳米颗粒沉积到生物膜上。
DOI：
10.1007/s10439-012-0626-0
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发表时间：
2013
影响因子：
3.8
通讯作者：
Cannon,CL
中科院分区：
文献类型：
--
作者：
Miller,JK;Neubig,R;Clemons,CB;Kreider,KL;Wilber,JP;Young,GW;Ditto,AJ;Yun,YH;Milsted,A;Badawy,HT;Panzner,MJ;Youngs,WJ;Cannon,CL
我们开发了纳米颗粒沉积并渗透到平行板流动池中生长的生物膜的数学模型。我们在流动池中进行沉积实验以支持建模。建模和实验的动机是聚合物纳米颗粒的潜在用途，作为杀死感染肺部深部通道的生物膜的治疗策略的一部分。在实验和模型中，携带聚合物纳米粒子的流体被注入平行板流动池中，其中生物膜在底板上生长。该模型由描述纳米粒子沉积和扩散的传输方程系统组成。应用利用流动池纵横比的标准渐近技术将模型简化为两个耦合偏微分方程。我们使用简化模型进行数值模拟。我们将实验观察结果与模拟结果进行比较，以估计生物膜中纳米颗粒的粘附系数和扩散系数。纳米粒子在生物膜厚度上的分布与扩散传输一致，并且在大约四个小时内实现了在厚度上的均匀分布。对于所考虑的低流速，纳米颗粒沉积似乎并未受到电池中流速的强烈影响。
We develop a mathematical model of nanoparticles depositing onto and penetrating into a biofilm grown in a parallel-plate flow cell. We carry out deposition experiments in a flow cell to support the modeling. The modeling and the experiments are motivated by the potential use of polymer nanoparticles as part of a treatment strategy for killing biofilms infecting the deep passages in the lungs. In the experiments and model, a fluid carrying polymer nanoparticles is injected into a parallel-plate flow cell in which a biofilm has grown over the bottom plate. The model consists of a system of transport equations describing the deposition and diffusion of nanoparticles. Standard asymptotic techniques that exploit the aspect ratio of the flow cell are applied to reduce the model to two coupled partial differential equations. We perform numerical simulations using the reduced model. We compare the experimental observations with the simulation results to estimate the nanoparticle sticking coefficient and the diffusion coefficient of the nanoparticles in the biofilm. The distributions of nanoparticles through the thickness of the biofilm are consistent with diffusive transport, and uniform distributions through the thickness are achieved in about four hours. Nanoparticle deposition does not appear to be strongly influenced by the flow rate in the cell for the low flow rates considered.