POSITIVE SELECTION OF ANTIGEN-SPECIFIC T-CELLS IN THYMUS BY RESTRICTING MHC MOLECULES
POSITIVE SELECTION OF ANTIGEN-SPECIFIC T-CELLS IN THYMUS BY RESTRICTING MHC MOLECULES
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DOI：
10.1038/335730a0
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发表时间：
1988-10-20
期刊：
影响因子：
64.8
通讯作者：
VONBOEHMER, H
中科院分区：
文献类型：
--
作者：
KISIELOW, P;TEH, HS;VONBOEHMER, H
胸腺来源的淋巴细胞 (T 细胞) 借助异二聚体 αβ T 细胞受体 (TCR)1,2 识别由主要组织相容性复合体 (MHC) 编码的 I 类或 II 类分子中的抗原。 T 细胞表面表达的 CD4 和 CDS 分子与 II 类和 I 类 MHC 分子的非多态性部分结合，协助 TCR 结合并可能参与信号传导 3,4。对 TCR 转基因小鼠 T 细胞发育的分析表明，T 细胞的 CD4/CD8 表型是由未成熟 CD4+8+ 胸腺细胞上表达的 αβ TCR 与胸腺 MHC 分子多态性结构域在没有标称抗原的情况下相互作用决定的。在这里，我们提供了直接证据，证明胸腺中抗原特异性、I 类 MHC 限制性 CD4−8+T 细胞的阳性选择需要 αβ TCR 与限制性 I 类 MHC 分子的特异性相互作用。
Thymus-derived lymphocytes (T cells) recognize antigen in the context of class I or class II molecules encoded by the major histocompatibility complex (MHC) by virtue of the heterodimeric αβ T-cell receptor (TCR)1,2. CD4 and CDS molecules expressed on the surface of T cells bind to nonpolymorphic portions of class II and class I MHC molecules and assist the TCR in binding and possibly in signalling3,4. The analysis of T-cell development in TCR transgenic mice has shown that the CD4/CD8 phenotype of T cells is determined by the interaction of the αβ TCR expressed on immature CD4+8+thymocytes with polymorphic domains of thymic MHC molecules in the absence of nominal antigen5. Here we provide direct evidence that positive selection of antigen-specific, class I MHC-restricted CD4−8+T cells in the thymus requires the specific interaction of the αβ TCR with the restricting class I MHC molecule.