New approaches using mass spectrometry to investigate changes to cytokinin and abscisic acid (ABA) concentrations in soil
New approaches using mass spectrometry to investigate changes to cytokinin and abscisic acid (ABA) concentrations in soil
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DOI：
10.1016/j.soilbio.2019.04.017
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发表时间：
2019-08
影响因子：
9.7
通讯作者：
Kirsty E. High;P. Ashton;M. Nelson;E. Rylott;J. Thomas-Oates;M. Hodson
中科院分区：
文献类型：
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作者：
Kirsty E. High;P. Ashton;M. Nelson;E. Rylott;J. Thomas-Oates;M. Hodson
植物激素如细胞分裂素、脱落酸（阿坝）和生长素在植物发育和调控过程中起着至关重要的作用。它们在植物中的作用是许多研究的焦点，研究使用质谱性能的最新进展，允许从植物组织中提取的低水平植物激素的定量。尽管取得了这些进展，但影响植物激素生产的外部因素研究较少。在这里，提出了一种新的方法，从植物生长介质（土壤和水培溶液）的范围内的植物激素的提取，其识别使用高质量精度质谱和随后的定量使用多反应监测（MRM）。检测植物组织以外的基质中的植物激素的能力提供了进一步研究诸如地下生物和土壤细菌等因素对植物激素产生的影响的机会。因此，将这种新的方法应用于植物生长介质，从一系列实验中比较存在和不存在蚯蚓的植物生长。一个小的，但显着的阿坝浓度增加，观察到蚯蚓的存在下，植物也存在时，甚至进一步增加。这一发现表明蚯蚓可以刺激植物阿坝的产生。本实验及其结果表明了研究植物组织外激素的价值，以及在这一领域进一步研究的潜在价值。
Phytohormones such as cytokinins, abscisic acid (ABA) and auxins play a vital role in plant development and regulatory processes. Their role within the plant is a focus for much research, with studies using recent advances in mass spectrometry performance allowing the quantification of low levels of phytohormones extracted from plant tissues. Despite these advances, external factors influencing the production of phytohormones are less well studied. Here, a new approach is presented for the extraction of a range of phytohormones from plant growth media (soil and hydroponic solution), their identification using high mass accuracy mass spectrometry and subsequent quantification using multiple reaction monitoring (MRM). The ability to detect phytohormones in matrices other than plant tissue presents the opportunity to study further the influence of factors such as below ground organisms and soil bacteria on phytohormone production.This novel approach was therefore applied to the plant growth media from a series of experiments comparing plant growth in the presence and absence of earthworms. A small but significant increase in ABA concentration was observed in the presence of earthworms, increasing even further when plants were also present. This finding suggests that earthworms could stimulate plant ABA production. This experiment and its outcomes demonstrate the value of studying phytohormones outside plant tissue, and the potential value of further research in this area.