The polymorphisms of Tim-1 promoter region are associated with rheumatoid arthritis in a Korean population
The polymorphisms of Tim-1 promoter region are associated with rheumatoid arthritis in a Korean population
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DOI：
10.1007/s00251-004-0743-5
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发表时间：
2005-01-01
期刊：
影响因子：
3.2
通讯作者：
Chung, HT
中科院分区：
文献类型：
--
作者：
Chae, SC;Park, YR;Chung, HT
已经确定T细胞免疫球蛋白结构域和粘蛋白结构域（TIM）蛋白家族在T细胞上表达。TIM家族的成员TIM-1被认为是与Th 2偏向的免疫应答的发展相关的膜蛋白，并且选择性地在Th 2细胞上表达。我们以前的研究表明，Tim-1的外显子4变异与过敏性疾病以及自身免疫性疾病如类风湿性关节炎（RA）的易感性相关。在这项研究中，我们评估了基因型和等位基因频率之间的关联Tim-1基因启动子区域，在RA患者和对照组没有RA，使用聚合酶链反应-限制性片段长度多态性和单碱基延伸的方法。我们进一步研究了RA患者中每个多态性基因型与C反应蛋白或类风湿因子水平之间的关系。RA组与非RA组比较，-1637A> G多态性基因型和等位基因频率差异有统计学意义（P= 0.0004和P= 0.001）。我们的研究结果强烈表明，在蒂姆-1启动子区的多态性可能与RA的易感性。
It has been determined that the family of T-cell immunoglobulin domain and mucin domain (TIM) proteins is expressed on T cells. A member of the TIM family, TIM-1, is considered to be a membrane protein associated with the development of Th2-biased immune responses and selectively expressed on Th2 cells. We previously showed that the exon 4 variations of Tim-1 are associated with susceptibility to allergic diseases, as well as autoimmune diseases such as rheumatoid arthritis ( RA). In this study, we assessed the association between genotype and allele frequencies of the Tim-1 gene promoter region, in both RA patients and the controls without RA, using polymerase chain reaction-restriction fragment length polymorphism and single-base extension methods. We further investigated the relationships among the genotypes of each polymorphism and C-reactive protein or rheumatoid factor levels in RA patients. The genotype and allele frequencies of the - 1637A> G polymorphism in RA patients are significantly different from those in the non-RA controls ( P= 0.0004 and P= 0.001, respectively). Our results strongly suggest that polymorphism in the Tim-1 promoter region might be associated with susceptibility to RA.