Specific expression of Olpiwi1 and Olpiwi2 in medaka (Oryzias latipes) germ cells.
Specific expression of Olpiwi1 and Olpiwi2 in medaka (Oryzias latipes) germ cells.
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DOI：
10.1016/j.bbrc.2011.12.062
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发表时间：
2012-02
影响因子：
3.1
通讯作者：
Haobin Zhao;J. Duan;Nana Cheng;Y. Nagahama
中科院分区：
文献类型：
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作者：
Haobin Zhao;J. Duan;Nana Cheng;Y. Nagahama
Piwi是果蝇生殖干细胞存活所必需的，其同源物已在多种生物中被发现。在这里，我们鉴定并表征了模式鱼medaka （Oryzias latipes）中piwi的两个同源基因Olpiwi1和Olpiwi2。Olpiwi1与斑马鱼中的Ziwi或小鼠中的Miwi相似，Olpiwi2与斑马鱼中的Zili或小鼠中的Mili相似。此外，Olpiwi2 mRNA是由两条不同的染色体产生的。RT-PCR结果显示，Olpiwi1和Olpiwi2主要在生殖腺中表达。原位杂交发现，在卵巢卵原细胞到成熟卵母细胞以及睾丸从精原细胞到精母细胞的整个卵母细胞发育过程中，Olpiwi1和Olpiwi2的生殖细胞特异性表达。RT-PCR和全座原位杂交表明，Olpiwi1和Olpiwi2均在母体胚胎中沉积。Olpiwi1和Olpiwi2在胚胎发育的原始生殖细胞中检测到。这些结果表明，Olpiwi1和Olpiwi2都是生殖细胞特异性的，可能在该模式物种的生殖细胞发育和配子体发生中发挥重要作用。
Piwi is necessary for germ stem cell survival in Drosophila and homologues have been identified in a diverse range of organisms. Here, we identify and characterize two homologous genes of piwi, Olpiwi1 and Olpiwi2, in the model fish medaka (Oryzias latipes). Olpiwi1 is similar to Ziwi in zebrafish or Miwi in the mouse, and Olpiwi2 is similar to Zili in zebrafish or Mili in the mouse. Moreover, Olpiwi2 mRNA is produced from two different chromosomes. RT-PCR showed expression of Olpiwi1 and Olpiwi2 predominantly in the gonads. In situ hybridization revealed germ cell-specific expression of Olpiwi1 and Olpiwi2 throughout the development of oocytes from oogonia to mature oocytes in the ovary, and from spermatogonia to spermatocytes in the testes of adults. RT-PCR and whole mount in situ hybridization showed that both Olpiwi1 and Olpiwi2 were maternally deposited in the embryo. Olpiwi1 and Olpiwi2 were detected in primordial germ cells during embryonic development. These results suggest that both Olpiwi1 and Olpiwi2 are germ cell specific, and may play important roles in germ cell development and gametogenesis in this model species.